Recombinant Human CRK

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935106861
info@markelab.com
name
Recombinant Human CRK
category
Proteins and Peptides
provider
FineTest
reference
P0259
tested applications
Western Blot, ELISA
Documents del producto
Instrucciones
Data sheet
Product specifications
| Category | Proteins and Peptides | 
| Host | E.Coli | 
| Reactivity | Human | 
| Assay Data | Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex. | 
| Recommended Dilution | ¥ | 
| Isotype | ¥ | 
| Clone ID | ¥ | 
| Observed MW | 57.7 kDa | 
| Expression | 41-304 | 
| Purity | Greater than 90% as determined by SDS-PAGE. | 
| Size 1 | 50μg | 
| Size 2 | 200μg | 
| Size 3 | 1mg | 
| Form | Lyophilized powder | 
| Tested Applications | Western Blot, ELISA | 
| Buffer | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4. | 
| Availability | 7 days | 
| Storage | The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle. | 
| UniProt ID | P46108 | 
| Alias | CRK, CRKII, p38, v-crk avian sarcoma virus CT10 oncogene homolog, CRK proto-oncogene | 
| Background | Protein CRK | 
| Status | RUO | 
| Note | Tag : N-terminal GST tag | 
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This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.
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Recombinant Human CRK
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