Rat NAD-Dependent Protein Deacetylase Sirtuin-3, Mitochondrial (SIRT3) Protein

Este producto es parte de SIRT3 - Sirtuin 3
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702€ (50 µg)

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935106861
info@markelab.com
name
Rat NAD-Dependent Protein Deacetylase Sirtuin-3, Mitochondrial (SIRT3) Protein
category
Proteins and Peptides
provider
Abbexa
reference
abx651441
tested applications
WB, SDS-PAGE

Description

Rat NAD-Dependent Protein Deacetylase Sirtuin-3, Mitochondrial (SIRT3) Protein (Active) is an active protein from Rat.

Documents del producto

Instrucciones
Data sheet
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Product specifications

Category
Proteins and Peptides
Immunogen Target
NAD-Dependent Protein Deacetylase Sirtuin-3, Mitochondrial (SIRT3)
Host
E. coli
Assay Type
Activity: Active
Sequence Fragment: Pro127-Glu303
Tag: N-terminal His tag
Origin
Rat
Conjugation
Unconjugated
Observed MW
Calculated MW: 20.8 kDa  Observed MW: 22 kDa
Expression
Recombinant
Purity
> 95%
Size 1
50 µg
Size 2
100 µg
Size 3
200 µg
Size 4
500 µg
Size 5
1 mg
Form
Lyophilized
Tested Applications
WB, SDS-PAGE
Buffer
Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300.
Availability
Shipped within 5-7 working days.
Storage
Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.
Dry Ice
No
UniProt ID
C6ZII9
Alias
SIR2L3,NAD-dependent protein deacetylase sirtuin-3 mitochondrial,SIR2-like protein 3,SIR2L3,hSIRT3
Background
Protein SIRT3
Status
RUO
Note
THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Concentration: Prior to lyophilization: 200 µg/ml

Descripción

SIRT3 is a NAD+-dependent deacetylase primarily localized in the mitochondria, where it plays a critical role in regulating mitochondrial metabolism, energy production, and oxidative stress response SIRT3 deacetylates and activates key mitochondrial enzymes, including acetyl-CoA synthetase 2 (ACSS2), superoxide dismutase 2 (SOD2), and components of the electron transport chain, enhancing ATP production, fatty acid oxidation, and antioxidant defense SIRT3 is essential for maintaining mitochondrial homeostasis during energy stress, such as fasting or caloric restriction, by stimulating pathways that improve energy efficiency and reduce reactive oxygen species (ROS) levels SIRT3 also promotes cellular survival under oxidative stress by deacetylating SOD2, which neutralizes superoxide radicals Dysregulation of SIRT3 is associated with aging, metabolic disorders, cardiovascular disease, and neurodegeneration, where impaired mitochondrial function leads to cellular damage and energy deficiency SIRT3 has tumor suppressor properties as it inhibits aerobic glycolysis (Warburg effect) in cancer cells by regulating key metabolic enzymes Its activation is being explored as a therapeutic strategy to combat mitochondrial dysfunction, improve metabolic health, and delay aging-related diseases

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