Rat High Mobility Group Protein B1 (HMGB1) Protein

category

Proteins and Peptides

origin

Rat

host

E. coli

immunogen target

High Mobility Group Protein B1 (HMGB1)

host

E. coli

conjugation

Unconjugated

form

Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.

tested applications

WB, SDS-PAGE

size 1

10 µg

size 2

50 µg

size 3

100 µg

size 4

200 µg

size 5

500 µg

observerd MW

Molecular Weight: Calculated MW: 26.2 kDa Observed MW (SDS-PAGE): 30 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Met1-Glu215 Tag: N-terminal His tag

expression

Recombinant

purity

> 90%

buffer

Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300.

storage

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.

or code

HMGB1

dry ice

No

availability

Shipped within 5-7 working days.

alias

HMG1,HMG3,HMG-1,SBP-1,Amphoterin

uniprot id

note

This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.