Rat Intestinal-Type Alkaline Phosphatase / IAP (ALPI) Protein

Product specifications

Proteins and Peptides

Intestinal-Type Alkaline Phosphatase / IAP (ALPI)

E. coli

Rat

Unconjugated

Concentration: Prior to lyophilization: 200 µg/ml
Sequence Fragment: Please enquire.
Tag: N-terminal His tag

Recombinant

> 90%

1 mg

5 mg

Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.

WB, SDS-PAGE

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.

Shipped within 1-2 months.

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.

No

IAP

Protein ALPI

RUO

This product is for research use only.   Not for human consumption, cosmetic, therapeutic or diagnostic use.

Descripción

Intestinal-type alkaline phosphatase (ALPI) is a glycoprotein enzyme primarily expressed on the luminal surface of intestinal epithelial cells, particularly in the small intestine. It plays a crucial role in maintaining gut homeostasis by detoxifying lipopolysaccharides (LPS), dephosphorylating inflammatory mediators, and promoting a healthy microbiota. ALPI is also involved in nutrient absorption, such as the hydrolysis of dietary phosphate esters, contributing to the regulation of phosphate metabolism. Its expression is upregulated in response to inflammation and microbial imbalance, acting as a protective mechanism against intestinal permeability and systemic endotoxemia. Additionally, ALPI prevents excessive immune activation by neutralizing pro-inflammatory signals, which is essential for preventing conditions such as inflammatory bowel disease (IBD) and sepsis. Alterations in ALPI expression or activity have been linked to gastrointestinal disorders, including Crohn's disease and ulcerative colitis. Its multifunctional role highlights its importance in gut health, immune modulation, and overall metabolic regulation.

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