RAC-Alpha Serine/Threonine-Protein Kinase Phospho-Thr450 (AKT1 pT450) Antibody
52€ (10 µg)
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935106861
info@markelab.com
name
RAC-Alpha Serine/Threonine-Protein Kinase Phospho-Thr450 (AKT1 pT450) Antibody
category
Primary Antibodies
provider
Abbexa
reference
abx012427
tested applications
ELISA, WB, IHC
Description
Rabbit polyclonal antibody against Akt1 protein. Immunogen region is C-terminal. Specificity is as follows for the reactive species: H:T450, M:T450, R:T450.
Documents del producto
Instrucciones
Data sheet
Product specifications
| Category | Primary Antibodies |
| Immunogen Target | RAC-Alpha Serine/Threonine-Protein Kinase Phospho-Thr450 (AKT1 pT450) |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Assay Data | Modification: Phosphorylation // Target Modification: Thr450 |
| Recommended Dilution | WB: 1/500 - 1/3000, IHC: 1/50 - 1/100, ELISA: 1/1000. Optimal dilutions/concentrations should be determined by the end user. |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Purification | Purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. |
| Size 1 | 10 µg |
| Size 2 | 100 µg |
| Size 3 | 200 µg |
| Size 4 | 300 µg |
| Size 5 | 1 mg |
| Form | Liquid |
| Tested Applications | ELISA, WB, IHC |
| Buffer | PBS (without Mg<sup>2+</sup> and Ca<sup>2+</sup>), pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% glycerol. |
| Availability | Shipped within 5-10 working days. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| UniProt ID | P31749 |
| Alias | AKT1,PKB,RAC,RAC-ALPHA |
| Background | Antibody anti-AKT1 |
| Status | RUO |
| Note | Concentration: 1 mg/ml - |
Descripción
The AKT (also known as Protein Kinase B) is a pivotal enzyme involved in regulating various cellular processes like metabolism, proliferation, survival, and growth. It acts downstream of phosphoinositide 3-kinase (PI3K) signaling pathway, which is activated by growth factors and cytokines. RAC-alpha serine/threonine-protein kinase is the full name of AKT. It belongs to the AGC (protein kinase A/protein kinase G/protein kinase C) family of kinases. AKT has three isoforms: AKT1, AKT2, and AKT3. Each isoform has slightly different tissue distributions and roles but shares the same basic structure and mechanism of action.Activation of AKT typically occurs through phosphorylation at two key residues: threonine 308 (Thr308) and serine 473 (Ser473). Once activated, AKT phosphorylates various downstream targets, including transcription factors, metabolic enzymes, and cell cycle regulators, ultimately leading to diverse cellular responses. Dysregulation of AKT signaling is implicated in various diseases, including cancer, diabetes, and cardiovascular diseases. Therefore, AKT has emerged as a significant target for therapeutic interventions in these conditions.
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