RAC-Alpha Serine/threonine-Protein Kinase Phospho-Ser473 (AKT1 pS473) Antibody
208€ (20 µl)
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935106861
info@markelab.com
name
RAC-Alpha Serine/threonine-Protein Kinase Phospho-Ser473 (AKT1 pS473) Antibody
category
Primary Antibodies
provider
Abbexa
reference
abx000192
tested applications
ELISA, WB, IHC
Description
AKT1 (pS473) Antibody is a Rabbit Polyclonal antibody against AKT1 (pS473). The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
Documents del producto
Instrucciones
Data sheet
Product specifications
| Category | Primary Antibodies |
| Immunogen Target | RAC-Alpha Serine/threonine-Protein Kinase Phospho-Ser473 (AKT1 pS473) |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Assay Data | Modification: Phosphorylation // Target Modification: Ser473 |
| Recommended Dilution | ELISA: 1 µg/ml, WB: 1/500 - 1/1000, IHC-P: 1/50 - 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Purification | Purified by affinity chromatography. |
| Size 1 | 20 µl |
| Size 2 | 100 µl |
| Size 3 | 2 × 100 µl |
| Form | Liquid |
| Tested Applications | ELISA, WB, IHC |
| Buffer | PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol. |
| Availability | Shipped within 5-10 working days. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| UniProt ID | P31749 |
| Gene ID | 207 |
| NCBI Accession | NP_005154.2 |
| Alias | AKT1,PKB,RAC,RAC-ALPHA |
| Background | Antibody anti-AKT1 |
| Status | RUO |
| Note | Concentration: > 0.2 mg/ml - |
Descripción
The AKT (also known as Protein Kinase B) is a pivotal enzyme involved in regulating various cellular processes like metabolism, proliferation, survival, and growth. It acts downstream of phosphoinositide 3-kinase (PI3K) signaling pathway, which is activated by growth factors and cytokines. RAC-alpha serine/threonine-protein kinase is the full name of AKT. It belongs to the AGC (protein kinase A/protein kinase G/protein kinase C) family of kinases. AKT has three isoforms: AKT1, AKT2, and AKT3. Each isoform has slightly different tissue distributions and roles but shares the same basic structure and mechanism of action.Activation of AKT typically occurs through phosphorylation at two key residues: threonine 308 (Thr308) and serine 473 (Ser473). Once activated, AKT phosphorylates various downstream targets, including transcription factors, metabolic enzymes, and cell cycle regulators, ultimately leading to diverse cellular responses. Dysregulation of AKT signaling is implicated in various diseases, including cancer, diabetes, and cardiovascular diseases. Therefore, AKT has emerged as a significant target for therapeutic interventions in these conditions.
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