Rabbit Anti-Sheep IgG H&L Antibody (HRP)

Secondary Antibodies

Target: Sheep IgG H&L

Rabbit

Sheep

Specificity: Based on immunoelectrophoresis, this product reacts with the heavy chains on sheep IgG and with light chains common to most sheep immunoglobulins. Non-reactive against non-immunoglobulin serum proteins. May cross-react with immunoglobulins from other species.
Concentration: Prior to lyophilization: 0.8 mg/ml
Reconstitute in 1.5 ml of distilled water. Centrifuge product if the solution is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a Pasteur pipette.
Centrifuge before opening to ensure complete recovery of vial contents.
Note: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

ELISA and WB (Chromogenic): 1/5000 - 1/100000, ELISA and WB (ECL): 1/10000 - 1/200000, IHC: 1/500 - 1/5000. Optimal dilutions/concentrations should be determined by the end user.

Polyclonal

HRP

Purified by immunoaffinity chromatography using antigens coupled to agarose beads.

1.5 ml

Lyophilized

ELISA, WB, IHC

Prior to lyophilization: 0.01 M Sodium phosphate, 0.25 M Sodium chloride, pH 7.6, containing 15 mg/ml BSA. Does not contain preservatives.

Shipped within 10-20 working days.

Store lyophilized at 2-8°C. After reconstitution, the product is stable for several weeks when stored undiluted at 2-8°C. For long-term storage after reconstitution, it is recommended to add an equal volume of glycerol to make a final glycerol concentration of 50%, then aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.

No

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.