Mouse Pregnancy Associated Plasma Protein A (PAPPA) Protein

234€ (10 µg)
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935106861
info@markelab.com
name
Mouse Pregnancy Associated Plasma Protein A (PAPPA) Protein
category
Proteins and Peptides
provider
Abbexa
reference
abx068655
tested applications
WB, SDS-PAGE
Description
Recombinant Pregnancy Associated Plasma Protein A (PAPPA) is a recombinant Mouse protein produced in a Prokaryotic expression system (E. coli).
Documents del producto
Instrucciones
Data sheet
Product specifications
Category | Proteins and Peptides |
Immunogen Target | Pregnancy Associated Plasma Protein A (PAPPA) |
Host | E. coli |
Origin | Mouse |
Conjugation | Unconjugated |
Observed MW | Molecular Weight: Calculated MW: 62.2 kDa Observed MW (SDS-PAGE): 60 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Phe999-Asn1272 Tag: N-terminal His tag and GST tag |
Expression | Recombinant |
Purity | > 95% |
Size 1 | 10 µg |
Size 2 | 50 µg |
Size 3 | 100 µg |
Size 4 | 200 µg |
Size 5 | 500 µg |
Form | Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
Tested Applications | WB, SDS-PAGE |
Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose. |
Availability | Shipped within 5-7 working days. |
Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
Dry Ice | No |
UniProt ID | Q8R4K8 |
Alias | PAPA; DIPLA1; PAPP-A; PAPPA1; ASBABP2; IGFBP-4ase |
Background | Protein PAPPA |
Status | RUO |
Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |
Descripción
PAPPA, also known as pregnancy-associated plasma protein-A, is a zinc-binding metalloproteinase that regulates the activity of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins (IGFBPs), specifically IGFBP-4 and IGFBP-5. This proteolytic activity increases the bioavailability of IGFs, which are crucial for cell growth, proliferation, and tissue repair. PAPPA is highly expressed during pregnancy, where it contributes to placental development and fetal growth, making it a key biomarker in prenatal screening for conditions like preeclampsia and Down syndrome. Outside of pregnancy, PAPPA is involved in regulating processes such as bone remodeling, wound healing, and inflammation. Elevated PAPPA expression has been observed in cardiovascular diseases and certain cancers, where it promotes IGF-mediated cell proliferation and tumor progression. Its role in modulating IGF signaling highlights its importance in both physiological and pathological conditions.
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