Mouse Pregnancy Associated Plasma Protein A (PAPPA) Protein

Este producto es parte de PAPPA - Pregnancy Associated Plasma Protein /Pappalysin
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234€ (10 µg)

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935106861
info@markelab.com
name
Mouse Pregnancy Associated Plasma Protein A (PAPPA) Protein
category
Proteins and Peptides
provider
Abbexa
reference
abx068655
tested applications
WB, SDS-PAGE

Description

Recombinant Pregnancy Associated Plasma Protein A (PAPPA) is a recombinant Mouse protein produced in a Prokaryotic expression system (E. coli).

Documents del producto

Instrucciones
Data sheet
Descargar

Product specifications

Category
Proteins and Peptides
Immunogen Target
Pregnancy Associated Plasma Protein A (PAPPA)
Host
E. coli
Origin
Mouse
Conjugation
Unconjugated
Observed MW
Molecular Weight: Calculated MW: 62.2 kDa  Observed MW (SDS-PAGE): 60 kDa

Concentration: Prior to lyophilization: 200 µg/ml

Sequence Fragment: Phe999-Asn1272

Tag: N-terminal His tag and GST tag
Expression
Recombinant
Purity
> 95%
Size 1
10 µg
Size 2
50 µg
Size 3
100 µg
Size 4
200 µg
Size 5
500 µg
Form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Tested Applications
WB, SDS-PAGE
Buffer
Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose.
Availability
Shipped within 5-7 working days.
Storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
Dry Ice
No
UniProt ID
Q8R4K8
Alias
PAPA; DIPLA1; PAPP-A; PAPPA1; ASBABP2; IGFBP-4ase
Background
Protein PAPPA
Status
RUO
Note
This product is for research use only.   Not for human consumption, cosmetic, therapeutic or diagnostic use.

Descripción

PAPPA, also known as pregnancy-associated plasma protein-A, is a zinc-binding metalloproteinase that regulates the activity of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins (IGFBPs), specifically IGFBP-4 and IGFBP-5. This proteolytic activity increases the bioavailability of IGFs, which are crucial for cell growth, proliferation, and tissue repair. PAPPA is highly expressed during pregnancy, where it contributes to placental development and fetal growth, making it a key biomarker in prenatal screening for conditions like preeclampsia and Down syndrome. Outside of pregnancy, PAPPA is involved in regulating processes such as bone remodeling, wound healing, and inflammation. Elevated PAPPA expression has been observed in cardiovascular diseases and certain cancers, where it promotes IGF-mediated cell proliferation and tumor progression. Its role in modulating IGF signaling highlights its importance in both physiological and pathological conditions.

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