Mouse Insulin Receptor Substrate 1 (IRS1) Protein
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935106861
info@markelab.com
Precio
221€ (10 µg)
Protein IRS1
proveedor
Abbexareference
abx067268Tested Applications
WB, SDS-PAGEreactivity
status
RUOclonality
Descripción
Mouse Insulin Receptor Substrate 1 (IRS1) is a recombinant Mouse protein produced in a Prokaryotic expression system (E. coli).
Background
IRS1 is a key adaptor protein that mediates insulin and insulin-like growth factor (IGF) signaling by interacting with the insulin receptor (IR) and IGF-1 receptor. Upon receptor activation, IRS1 is phosphorylated on tyrosine residues, recruiting downstream effectors such as PI3K and Grb2 to activate pathways like PI3K/Akt and Ras/MAPK. IRS1 regulates glucose uptake, metabolism, cell growth, and survival, playing a central role in insulin signaling. It is highly expressed in insulin-sensitive tissues like muscle, liver, and adipose. Dysregulation of IRS1 is associated with insulin resistance, obesity, type 2 diabetes, and cancer, where aberrant signaling promotes proliferation and survival. Knockout studies show growth retardation, metabolic abnormalities, and impaired glucose homeostasis, underscoring its importance in metabolism and cellular signaling.
Características del producto
category
Proteins and Peptides
origin
Mouse
host
E. coli
immunogen target
Insulin Receptor Substrate 1 (IRS1)
host
E. coli
conjugation
Unconjugated
form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
tested applications
WB, SDS-PAGE
size 1
10 µg
size 2
50 µg
size 3
100 µg
size 4
200 µg
size 5
500 µg
observerd MW
Molecular Weight: Calculated MW: 31.2 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Pro837-Gln1089 Tag: N-terminal His tag
expression
Recombinant
purity
> 95%
buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
or code
IRS1
dry ice
No
availability
Shipped within 5-7 working days.
alias
IRS1, HIRS-1, insulin receptor substrate 1
uniprot id
note
This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
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