Mouse High Mobility Group Protein B1 (HMGB1) Protein

category

Proteins and Peptides

origin

Mouse

host

E. coli

immunogen target

High Mobility Group Protein B1 (HMGB1)

host

E. coli

conjugation

Unconjugated

form

Lyophilized Reconstitute in ddH2O to a concentration of 0.1-0.25 mg/ml. Do not vortex.

tested applications

WB, SDS-PAGE

size 1

10 µg

size 2

50 µg

size 3

100 µg

size 4

200 µg

size 5

500 µg

observerd MW

Molecular Weight: Calculated MW: 26.2 kDa Observed MW: 33 kDa (determined by SDS-PAGE) Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. Concentration: Prior to lyophilization: 50 µg/ml Sequence Fragment: Met1-Glu215 Tag: N-terminal His tag

expression

Recombinant

purity

> 90%

buffer

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose.

storage

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.

or code

HMGB1

dry ice

No

availability

Shipped within 5-7 working days.

alias

HMG1,HMG3,HMG-1,SBP-1,Amphoterin

uniprot id

note

This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.