Mouse Damage Specific DNA Binding Protein 2 (DDB2) Protein
234€ (10 µg)
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Name
Mouse Damage Specific DNA Binding Protein 2 (DDB2) Protein
Category
Proteins and Peptides
Provider
Abbexa
Reference
abx166155
Tested Applications
WB, SDS-PAGE
Description
Mouse Damage Specific DNA Binding Protein 2 is a recombinant Mouse protein expressed in E. coli.
Documentos del producto
Instrucciones
Data sheet
Especificaciones del producto
| Category | Proteins and Peptides |
| Immunogen Target | Damage Specific DNA Binding Protein 2 (DDB2) |
| Host | E. coli |
| Assay Type | Activity: Not tested Sequence Fragment: Lys212-Leu407 Tag: N-terminal His tag |
| Origin | Mouse |
| Conjugation | Unconjugated |
| Observed MW | Calculated MW: 26.0 kDa |
| Expression | Recombinant |
| Purity | > 95% |
| Size 1 | 10 µg |
| Size 2 | 50 µg |
| Size 3 | 100 µg |
| Size 4 | 200 µg |
| Size 5 | 500 µg |
| Form | Lyophilized |
| Tested Applications | WB, SDS-PAGE |
| Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300. |
| Availability | Shipped within 5-7 working days. |
| Storage | Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles. |
| Dry Ice | No |
| UniProt ID | Q99J79 |
| Alias | XPE,DDBB,UV-DDB2,UV-damaged DNA-binding protein 2,Damage-specific DNA-binding protein 2,DDB p48 subunit |
| Background | Protein DDB2 |
| Status | RUO |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. Concentration: Prior to lyophilization: 200 µg/ml |
Background
Damage-specific DNA binding protein 2 (DDB2) is a substrate recognition protein that works in concert with DDB1 within the UV-DDB complex, a subset of the CUL4-DDB1 E3 ubiquitin ligase system. DDB2 plays an essential role in nucleotide excision repair (NER) by recognizing UV-induced DNA damage, such as cyclobutane pyrimidine dimers (CPDs) and 6-4 photoproducts, and recruiting repair factors to the damage site. Its high affinity for damaged DNA and ability to induce chromatin remodeling are key to facilitating repair processes. DDB2 also mediates the ubiquitination and degradation of histones and other chromatin-associated proteins, promoting access to damaged DNA. Mutations or dysregulation of DDB2 have been linked to xeroderma pigmentosum group E (XP-E), a condition characterized by extreme sensitivity to UV light and a high predisposition to skin cancer. Beyond DNA repair, DDB2 is implicated in cell cycle regulation and apoptosis, highlighting its multifunctional role in maintaining genomic integrity.
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