DNA Damage-Binding Protein 2 (DDB2) Antibody
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Description
DDB2 Antibody is a Rabbit Polyclonal antibody against DDB2. This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. This subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities. Two transcript variants encoding different isoforms have been found for this gene.
Documents del producto
Product specifications
| Category | Primary Antibodies |
| Immunogen Target | DNA Damage-Binding Protein 2 (DDB2) |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Recommended Dilution | ELISA: 1 µg/ml, WB: 1/500 - 1/2000, IF/ICC: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user. |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Purification | Purified by affinity chromatography. |
| Size 1 | 20 µl |
| Size 2 | 100 µl |
| Size 3 | 2 × 100 µl |
| Form | Liquid |
| Tested Applications | ELISA, WB, IF/ICC |
| Buffer | PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol. |
| Availability | Shipped within 5-10 working days. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| UniProt ID | Q92466 |
| Gene ID | 1643 |
| NCBI Accession | NP_000098.1 |
| Alias | XPE,DDBB,UV-DDB2,UV-damaged DNA-binding protein 2,Damage-specific DNA-binding protein 2,DDB p48 subunit |
| Background | Antibody anti-DDB2 |
| Status | RUO |
| Note | Concentration: > 0.2 mg/ml - |
Descripción
Damage-specific DNA binding protein 2 (DDB2) is a substrate recognition protein that works in concert with DDB1 within the UV-DDB complex, a subset of the CUL4-DDB1 E3 ubiquitin ligase system. DDB2 plays an essential role in nucleotide excision repair (NER) by recognizing UV-induced DNA damage, such as cyclobutane pyrimidine dimers (CPDs) and 6-4 photoproducts, and recruiting repair factors to the damage site. Its high affinity for damaged DNA and ability to induce chromatin remodeling are key to facilitating repair processes. DDB2 also mediates the ubiquitination and degradation of histones and other chromatin-associated proteins, promoting access to damaged DNA. Mutations or dysregulation of DDB2 have been linked to xeroderma pigmentosum group E (XP-E), a condition characterized by extreme sensitivity to UV light and a high predisposition to skin cancer. Beyond DNA repair, DDB2 is implicated in cell cycle regulation and apoptosis, highlighting its multifunctional role in maintaining genomic integrity.
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anti- DDB2 antibody
Required for DNA repair. Binds to DDB1 to form the UV-damaged DNA-binding protein complex(the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway(the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers(CPD), 6-4 photoproducts(6-4 PP), apurinic sites and short mismatches. Also appears to function as the substrate recognition module for the DCX(DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB1-CUL4-ROC1(also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB1-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB1-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. Isoform D1 and isoform D2 inhibit UV-damaged DNA repair.
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DNA Damage-Binding Protein 2 (DDB2) Antibody
DDB2 Antibody is a Rabbit Polyclonal antibody against DDB2. This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. This subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities. Two transcript variants encoding different isoforms have been found for this gene.
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