Mouse Apolipoprotein B-100 (APOB) Protein
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935106861
info@markelab.com
Precio
208€ (10 µg)
Protein APOB
proveedor
Abbexareference
abx652546Tested Applications
WB, SDS-PAGEreactivity
status
RUOclonality
Descripción
Mouse Apolipoprotein B-100 (APOB) Protein is a Recombinant Mouse protein expressed in E. coli.
Background
APOB is a major structural protein of low-density lipoproteins (LDL), very-low-density lipoproteins (VLDL), and chylomicrons, which are essential for lipid transport and metabolism in the body It exists in two primary isoforms: APOB-100, produced in the liver, and APOB-48, synthesized in the intestine APOB-100 is critical for the assembly and secretion of VLDL and LDL particles, facilitating the transport of cholesterol and triglycerides to peripheral tissues via binding to the LDL receptor (LDLR) Mutations or overexpression of APOB contribute to familial hypercholesterolemia and other dyslipidemias, leading to elevated LDL cholesterol levels and increased risk of atherosclerosis and cardiovascular diseases APOB-48, on the other hand, plays a key role in chylomicron formation and dietary fat absorption Genetic variations in the APOB gene have been linked to metabolic disorders, including obesity, diabetes, and fatty liver disease APOB levels serve as a marker of cardiovascular risk, with elevated concentrations indicating higher susceptibility to coronary artery disease Therapies targeting APOB, such as antisense oligonucleotides or LDL-lowering drugs like statins, have shown significant success in reducing cardiovascular events APOB remains a key focus in lipid biology due to its central role in lipid transport and cardiovascular health
Características del producto
category
Proteins and Peptides
origin
Mouse
host
E. coli
immunogen target
Apolipoprotein B-100 (APOB)
host
E. coli
conjugation
Unconjugated
form
Lyophilized Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/ml. Do not vortex.
tested applications
WB, SDS-PAGE
size 1
10 µg
size 2
50 µg
size 3
100 µg
size 4
200 µg
size 5
500 µg
observerd MW
Molecular Weight: Calculated MW: 44.7 kDa Observed MW (SDS-PAGE): 50 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Leu32-Met306 Tag: N-terminal His tag and SUMO tag
expression
Recombinant
purity
> 95%
buffer
Prior to lyophilization: 100 mM NaHCO<sub>3</sub>, 500 mM NaCl, pH 8.3, containing 0.01% Sarcosyl, 5% Trehalose.
storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
or code
APOB
dry ice
No
availability
Shipped within 5-7 working days.
alias
APOB, FLDB, LDLCQ4, apoB-100,apolipoprotein B, FCHL2
uniprot id
note
This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
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