Mouse and Rat Growth Hormone (m/rGH) ELISA
510€ (96 det.)
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935106861
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name
Mouse and Rat Growth Hormone (m/rGH) ELISA
category
ELISA Kits
provider
Mediagnost
reference
E023
tested applications
Elisa
Description
E023 ELISA is intended to be used for the measurement of Growth Hormone in mouse and rat serum and plasma samples for research use.
Documents del producto
Instrucciones
Data sheet
Product specifications
| Category | ELISA Kits |
| Reactivity | Mouse,Rat |
| Assay Data | Reference Material: recombinant rat growth hormone Incubation Period: 3h Calibrator: recombinant rat growth hormone Controls: 2 Control sera, lyophilized |
| Assay Type | Sandwich |
| Test Range | 0.04 – 45 µg/L |
| Sensitivity | ∅ ≤ 0.04 µg/L |
| Recommended Dilution | 1:5 |
| Origin | Antibody: Goat / Guinea pig |
| Size 1 | 96 det. |
| Tested Applications | Elisa |
| Sample Type | Serum EDTA / Heparin Plasma |
| Availability | 3-5 days. |
| Storage | Shelf life: between 1 and 2 years Store the kit at 2-8°C after receipt until its expiry date |
| Alias | Growth hormone,GH, somatotropin |
| Background | Elisa Kits for: GH1 |
| Status | RUO |
| Note | Intra/Interassay Variance [%]∅ ≤ 10 |
Descripción
E Kit E023 está diseñado para medir la hormona del crecimiento en muestras de suero y plasma de ratones y ratas para uso en investigación.
The Mediagnost m/rGH ELISA, E023 is a so-called sandwich-assay. It utilizes two different specific high affinity polyclonal antibodies for this protein. The GH in the samples binds quantitatively to the immobilized antibody. In the following step, the biotinylated antibody in turn binds GH. After washing, a streptavidin-peroxidase-enzyme conjugate will be added, which will bind highly specific to the biotin of the antibody. Subsequently, the peroxidase catalyzes an enzymatic reaction resulting in a blue coloration. The intensity of the blue color depends on the GH content of the sample. The reaction is stopped by the addition of stop solution and color intensity is quantified by measuring the absorption.
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