Mouse 3-oxo-5-beta-steroid 4-dehydrogenase (AKR1D1) ELISA Kit

Product specifications

ELISA Kits

3-oxo-5-beta-steroid 4-dehydrogenase (AKR1D1)

Mouse

Colorimetric

Quantitative

0.156 ng/ml - 10 ng/ml

Optimal dilutions/concentrations should be determined by the end user.

96 tests

Lyophilized

ELISA

Tissue homogenates, cell lysates and other biological fluids.

Shipped within 5-15 working days. The validity for this kit is 6 months.

Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

No

Q8VCX1

208665

AKR1D1,SRD5B1

Elisa kits for AKR1D1

RUO

Validity: The validity for this kit is 6 months.

This product is for research use only.   The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments.   Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.

Descripción

AKR1D1, also known as Δ4-3-ketosteroid-5β-reductase, is a liver-specific enzyme that plays a pivotal role in bile acid synthesis and steroid hormone metabolism. It catalyzes the reduction of double bonds in Δ4-3-ketosteroids, converting cortisol, aldosterone, and other steroid precursors into their inactive 5β-reduced metabolites. This activity is essential for bile acid production, as AKR1D1 mediates the transformation of cholesterol-derived intermediates into bile acids, which are critical for lipid digestion and cholesterol homeostasis. AKR1D1 deficiency results in bile acid synthesis disorders, leading to cholestasis, liver dysfunction, and fat malabsorption. Additionally, AKR1D1 contributes to steroid clearance and inactivates glucocorticoids, regulating cortisol levels and preventing glucocorticoid excess. Dysregulation of AKR1D1 is linked to liver diseases, such as non-alcoholic fatty liver disease (NAFLD) and cirrhosis, where impaired bile acid synthesis disrupts lipid metabolism. It is also implicated in metabolic syndrome and hormone-driven disorders due to its role in steroid hormone regulation.

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The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta (4) 3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. Three transcript variants encoding different isoforms have been found for this gene. Other variants may be present, but their full-length natures have not been determined yet.

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