Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) Protein (Active)

Proteins and Peptides

Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT)

E. coli

Activity: Active
Biological Activity: One unit is defined as the amount of enzyme required to catalyze the incorporation of 1 nmol of deoxyribonucleotide into acid-insoluble forms in 10 minutes at 37 °C, using poly(A)-oligo(dT)12-18 as the template-primer. Standard cDNA Synthesis Conditions: 50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl2, 10 mM DTT, 1.0 mM each dATP, dGTP, dCTP, and dTTP; 0.2 mg random hexamer, 1-5 mg RNA, 200 units MMLV-RT. The reaction volume was 20 ml and incubation was carried out at 42 °C for 45 min.

Optimal dilutions/concentrations should be determined by the end user.

Virus

Recombinant

2500 U

10 kU

50 kU

Liquid

50 mM Tris-HCl, 0.1 M NaCl, 0.1% Triton X-100, 2 mM DTT, 0.1 mM EDTA and 50% glycerol.

Shipped within 5-10 working days.

Store at 10°C for up to 5 days, or aliquot and store at -20 °C. Avoid repeated freezing/thawing cycles.

No

Protein MMLV-RT

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Concentration: 200 U/µl