Large T Antigen (LT) Protein

Proteins and Peptides

Large T Antigen (LT)

E. coli

General

Unconjugated

Molecular Weight: Calculated MW: 28.8 kDa
Observed MW (SDS-PAGE): 33 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Concentration: Prior to lyophilization: 200 µg/ml
Sequence Fragment: Lys131-Glu260
Tag: N-terminal His tag and SUMO tag

Recombinant

> 95%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/ml. Do not vortex.

WB, SDS-PAGE

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose.

Shipped within 5-7 working days.

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.

No

P03070

Protein LT

RUO

This product is for research use only.   Not for human consumption, cosmetic, therapeutic or diagnostic use.