KD Plus DNA Polymerase Enzyme (with 2.5 mM dNTPs)

Proteins and Peptides

KD Plus DNA Polymerase

Unconjugated

100 U

200 U

1200 U

PCR

Enzyme Buffer: 50 mM Tris-Cl (pH 8.0), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, Stabilizers, 50% glycerol. <br> KD Plus Buffer with 5 mM MgSO<sub>4</sub>: Contains 100 mM Tris-Cl (pH 9.2), 50 mM NH<sub>4</sub>SO<sub>4</sub>, 200 mM KCl, 5 mM MgSO<sub>4</sub> and 10% glycerol.

Shipped within 10-20 working days.

Store at -20 °C. Avoid repeated freeze/thaw cycles.

No

Protein KD Plus DNA Polymerase

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Reaction Components:    Component  Volume  Final Concentration    Template  Variable  as required    Forward Primer (10 µM)  1 µl  0.2 µM   Reverse Primer (10 µM)  1 µl  0.2 µM 5X KD Plus Buffer  10 µl  1X 2.5 mM dNTPs 4 µl  0.2 mM  KD Plus DNA Polymerase 0.1 µl  1 U   Nuclease-free H2O  Variable N/A  Total Volume  50 µl N/A    Thermal Cycling Conditions:     Number of Cycles  Temperature  Time  1 cycle 94 °C  2-5 min  30-25 cycles  94 °C 30 seconds 50-60 °C 30 seconds 72 °C 1 kb/min 1 cycle 72 °C5-10 min