Human X-Ray Repair Cross Complementing 5/6 Heterodimer (XRCC5-XRCC6) Protein
1118€ (50 µg)
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name
Human X-Ray Repair Cross Complementing 5/6 Heterodimer (XRCC5-XRCC6) Protein
category
Proteins and Peptides
provider
Abbexa
reference
abx693934
tested applications
SDS-PAGE
Description
Human XRCC5 & XRCC6 Heterodimer Protein is a recombinant Human protein expressed in Baculovirus-Insect Cells. A DNA sequence encoding the XRCC5 (P13010) (Met 1-Ile 732) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the XRCC6 (P12956) (Met 1-Asp 609) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
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Data sheet
Product specifications
| Category | Proteins and Peptides |
| Immunogen Target | X-Ray Repair Cross Complementing 5/6 Heterodimer (XRCC5-XRCC6) |
| Host | Insect |
| Assay Type | Activity: Not tested Sequence Fragment: XRCC5 (Met1-Ile732) and XRCC6 (Met1-Asp609) Tag: N-terminal His tag |
| Origin | Human |
| Observed MW | Calculated MW: 72/85 kDa Observed MW (SDS-PAGE): 70/85 kDa |
| Expression | Recombinant |
| Purity | > 90% (SDS-PAGE) |
| Size 1 | 50 µg |
| Form | Lyophilized |
| Tested Applications | SDS-PAGE |
| Buffer | Prior to lyophilization: 20 mM Tris, 500 mM NaCl, pH 8.0, containing 2.85% Trehalose, 10.07% Mannitol, 0.005% Tween-80 and 10% glycerol. |
| Availability | Shipped within 5-15 working days. |
| Storage | Storage: Store lyophilized between -20 °C and -80°C. Stability: Stable when stored reconstituted at 2-8°C for up to 1 week. Reconstituted aliquots are stable at -20°C for up to 3 months. Shelf Life: 12 months. |
| Dry Ice | No |
| UniProt ID | P13010, P12956 |
| Gene ID | 7520, 2547 |
| OMIM | 194364, 152690 |
| Background | Protein XRCC5-XRCC6 |
| Status | RUO |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. Reconstitute in sterile distilled water to a concentration of 0.14 mg/ml. Do not vortex. Endotoxin Level: < 1.0 EU per µg (LAL method). Centrifuge before opening to ensure complete recovery of vial contents. |
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Human X-Ray Repair Cross Complementing 5/6 Heterodimer (XRCC5-XRCC6) Protein
Human XRCC5 & XRCC6 Heterodimer Protein is a recombinant Human protein expressed in Baculovirus-Insect Cells. A DNA sequence encoding the XRCC5 (P13010) (Met 1-Ile 732) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the XRCC6 (P12956) (Met 1-Asp 609) was fused with a polyhistidine tag at the N-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
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