Human Slit Homolog 3 (SLIT3) Protein

Human Slit Homolog 3 (SLIT3) Protein
234€ (10 µg)

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Name
Human Slit Homolog 3 (SLIT3) Protein
Category
Proteins and Peptides
Provider
Abbexa
Reference
abx069124
Tested Applications
WB, SDS-PAGE

Description

Human Slit Homolog 3 (SLIT3) is a recombinant Human protein expressed in E. coli.

Documentos del producto

Instrucciones
Data sheet
Descargar

Especificaciones del producto

Category
Proteins and Peptides
Immunogen Target
Slit Homolog 3 (SLIT3)
Host
E. coli
Assay Type
Activity: Not tested
Sequence Fragment: Lys1405-Glu1512
Tag: N-terminal His tag
Origin
Human
Conjugation
Unconjugated
Observed MW
Calculated MW: 18.6 kDa  Observed MW (SDS-PAGE): 22 kDa  Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Expression
Recombinant
Purity
> 97%
Size 1
10 µg
Size 2
50 µg
Size 3
100 µg
Size 4
200 µg
Size 5
500 µg
Form
Lyophilized
Tested Applications
WB, SDS-PAGE
Buffer
Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose.
Availability
Shipped within 5-7 working days.
Storage
Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.
Dry Ice
No
UniProt ID
O75094
Gene ID
6586
OMIM
603745
Alias
KIAA0814,MEGF5,SLIL2,
Background
Protein SLIT3
Status
RUO
Note
THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Concentration: Prior to lyophilization: 250 µg/ml

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