Human Pyruvate Dehydrogenase Alpha (PDHa) Protein

Proteins and Peptides

Pyruvate Dehydrogenase Alpha (PDHa)

E. coli

Human

Unconjugated

Molecular Weight: Calculated MW: 41.5 kDa  Observed MW (SDS-PAGE): 46 kDa  Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.

Concentration: Prior to lyophilization: 1000 µg/ml

Sequence Fragment: Phe30-Ser390

Tag: N-terminal His tag

Recombinant

> 90%

10 µg

50 µg

100 µg

200 µg

500 µg

Lyophilized Reconstitute in dH2O. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.

WB, SDS-PAGE

Prior to lyophilization: 100mM NaHCO<sub>3</sub>, 500mM NaCl, pH 8.3, containing 0.01% Sarcosyl and 5% Trehalose.

Shipped within 5-7 working days.

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.

No

P08559

Protein PDHa

RUO

This product is for research use only.   Not for human consumption, cosmetic, therapeutic or diagnostic use.