Human Protein Regulator of Cytokinesis 1 (PRC1) Protein
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Description
Human PRC1 Protein is a recombinant protein from Human produced in Baculovirus-Insect Cells. A DNA sequence encoding the human PRC1 isoform 1 (NP_003972.1) (Met 1-Ser 620) was expressed, with a polyhistidine tag at the N-terminus.
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Product specifications
| Category | Proteins and Peptides |
| Immunogen Target | PRC1 |
| Host | Insect |
| Assay Type | Activity: Not tested Sequence Fragment: Met1-Ser620 Tag: N-terminal His tag |
| Origin | Human |
| Observed MW | 74 kDa |
| Expression | Recombinant |
| Purity | > 95% (SDS-PAGE) |
| Size 1 | 50 µg |
| Form | Lyophilized |
| Tested Applications | SDS-PAGE |
| Buffer | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 20% gly, 3mM DTT. |
| Availability | Shipped within 5-15 working days. |
| Storage | Storage: Store lyophilized between -20 °C and -80°C. Stability: Stable when stored reconstituted at 2-8°C for up to 1 week. Reconstituted aliquots are stable at -20°C for up to 3 months. Shelf Life: 12 months. |
| Dry Ice | No |
| NCBI Accession | NP_003972.1 |
| Background | Protein PRC1 |
| Status | RUO |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. Endotoxin Level: < 1.0 EU per µg (LAL method). |
Descripción
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Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression(PubMed:17409436).
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