Human Numb Homolog (NUMB) Protein

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935106861
info@markelab.com
Precio
234€ (10 µg)
Protein NUMB
proveedor
Abbexareference
abx068374Tested Applications
WB, SDS-PAGEreactivity
status
RUOclonality
Descripción
Numb Homolog (NUMB) is a recombinant Human protein produced in a Prokaryotic expression system (E. coli).
Background
NUMB endocytic adaptor protein (NUNB) plays a critical role in cell fate determination, particularly in neural progenitor cells, where it antagonizes Notch signaling pathways to promote differentiation and prevent excessive self-renewal. It functions as an adaptor protein in clathrin-mediated endocytosis, facilitating the internalization of membrane receptors and influencing their downstream signaling pathways, including Notch and integrins. The asymmetrical distribution of NUMB during mitosis determines daughter cell fate by regulating receptor-mediated signaling and is crucial for proper neural development. NUMB also interacts with various proteins such as alpha-adaptin, EPS15, and integrins, contributing to cellular processes like adhesion, migration, and polarity. Aberrations in NUMB expression or function have been implicated in cancers, including breast cancer and glioblastoma, where its loss correlates with enhanced Notch signaling and increased tumorigenic potential. In addition to its canonical roles, NUMB has been found to influence p53 stability and activity, further linking it to tumor suppression and apoptosis regulation. Its localization and function are tightly regulated through post-translational modifications, including phosphorylation by kinases like LATS and Aurora A, which affect its stability and interaction partners.
Características del producto
category
Proteins and Peptides
origin
Human
host
E. coli
immunogen target
Numb Homolog (NUMB)
host
E. coli
conjugation
Unconjugated
form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
tested applications
WB, SDS-PAGE
size 1
10 µg
size 2
50 µg
size 3
100 µg
size 4
200 µg
size 5
500 µg
observerd MW
Molecular Weight: Calculated MW: 15.0 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Leu376-Phe482 Tag: N-terminal His tag
expression
Recombinant
purity
> 95%
buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
or code
NUMB
dry ice
No
availability
Shipped within 5-7 working days.
alias
NUMB, C14orf41, S171, c14_5527, endocytic adaptor protein, NUMB endocytic adaptor protein
uniprot id
note
This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
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