Human Numb Homolog (NUMB) Protein

Human Numb Homolog (NUMB) Protein

Pida mas información

935106861

info@markelab.com

Precio

234€ (10 µg)

Protein NUMB

proveedor

Abbexa

reference

abx068374

Tested Applications

WB, SDS-PAGE

reactivity

status

RUO

clonality

Descripción

Numb Homolog (NUMB) is a recombinant Human protein produced in a Prokaryotic expression system (E. coli).


Background

NUMB endocytic adaptor protein (NUNB) plays a critical role in cell fate determination, particularly in neural progenitor cells, where it antagonizes Notch signaling pathways to promote differentiation and prevent excessive self-renewal. It functions as an adaptor protein in clathrin-mediated endocytosis, facilitating the internalization of membrane receptors and influencing their downstream signaling pathways, including Notch and integrins. The asymmetrical distribution of NUMB during mitosis determines daughter cell fate by regulating receptor-mediated signaling and is crucial for proper neural development. NUMB also interacts with various proteins such as alpha-adaptin, EPS15, and integrins, contributing to cellular processes like adhesion, migration, and polarity. Aberrations in NUMB expression or function have been implicated in cancers, including breast cancer and glioblastoma, where its loss correlates with enhanced Notch signaling and increased tumorigenic potential. In addition to its canonical roles, NUMB has been found to influence p53 stability and activity, further linking it to tumor suppression and apoptosis regulation. Its localization and function are tightly regulated through post-translational modifications, including phosphorylation by kinases like LATS and Aurora A, which affect its stability and interaction partners.

Características del producto

category

Proteins and Peptides


origin

Human


host

E. coli


immunogen target

Numb Homolog (NUMB)


host

E. coli


conjugation

Unconjugated


form

Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.


tested applications

WB, SDS-PAGE


size 1

10 µg


size 2

50 µg


size 3

100 µg


size 4

200 µg


size 5

500 µg


observerd MW

Molecular Weight: Calculated MW: 15.0 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Leu376-Phe482 Tag: N-terminal His tag


expression

Recombinant


purity

> 95%


buffer

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.


storage

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.


or code

NUMB


dry ice

No


availability

Shipped within 5-7 working days.


alias

NUMB, C14orf41, S171, c14_5527, endocytic adaptor protein, NUMB endocytic adaptor protein


note

This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.


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