Human Mitogen-Activated Protein Kinase 1 / ERK2 (MAPK1) Protein

Proteins and Peptides

Mitogen-Activated Protein Kinase 1 / ERK2 (MAPK1)

E. coli

Activity: Active
Biological Activity: STAT3 has been identified as an interactor of MAPK1, therefore a binding ELISA assay was conducted to detect the interaction of recombinant Human MAPK1 and recombinant Human STAT3. Briefly, MAPK1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to STAT3-coated microplate wells and incubated for 2 h at 37 °C. Wells were washed with PBST and incubated for 1 h with anti-MAPK1 polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, the wells were aspirated and washed 3 times. TMB substrate solution was added to the wells, which were then incubated for 15-25 minutes at 37°C. Finally, 50 µl stop solution was added to the wells and the absorbance was measured at 450 nm immediately. The binding activity of MAPK1 and STAT3 is shown in Figure 3, the EC50 is 1.06 µg/ml.
Sequence Fragment: Tyr25-Ser360
Tag: N-terminal His tag

Human

Unconjugated

Calculated MW: 42.8 kDa  Observed MW: 43 kDa (determined by SDS-PAGE)

Recombinant

> 95%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized

WB, SDS-PAGE

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl and 5% Trehalose.

Shipped within 5-7 working days.

Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.

No

P28482

Protein MAPK1

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Endotoxin Level: < 1.0 EU per 1 µg (LAL method)
Concentration: Prior to lyophilization: 1000 µg/ml