Human Mitogen-Activated Protein Kinase 1 / ERK2 (MAPK1) Protein

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Description
Recombinant Extracellular Signal Regulated Kinase 2 (ERK2) is an active Human protein produced in a Prokaryotic expression system (E. coli).
Documents del producto
Product specifications
Category | Proteins and Peptides |
Immunogen Target | Mitogen-Activated Protein Kinase 1 / ERK2 (MAPK1) |
Host | E. coli |
Origin | Human |
Conjugation | Unconjugated |
Observed MW | Molecular Weight: Calculated MW: 42.8 kDa Observed MW: 43 kDa (determined by SDS-PAGE) Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Tyr25-Ser360 Tag: N-terminal His tag |
Expression | Recombinant |
Purity | > 95% |
Size 1 | 50 µg |
Size 2 | 100 µg |
Size 3 | 200 µg |
Size 4 | 500 µg |
Size 5 | 1 mg |
Form | Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
Tested Applications | WB, SDS-PAGE |
Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.05% Sarcosyl and 5% Trehalose. |
Availability | Shipped within 5-7 working days. |
Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
Dry Ice | No |
UniProt ID | P28482 |
Background | Protein MAPK1 |
Status | RUO |
Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |
Descripción
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MAPK1 Antibody is a Rabbit Polyclonal antibody against MAPK1. This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
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