Human Lys-63-Specific Deubiquitinase BRCC36 (BRCC3) Protein
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935106861
info@markelab.com
Precio
208€ (10 µg)
Protein BRCC3
proveedor
Abbexareference
abx650811Tested Applications
WB, SDS-PAGEreactivity
status
RUOclonality
Descripción
Human Lys-63-Specific Deubiquitinase BRCC36 (BRCC3) Protein is a recombinant Human protein produced in a Prokaryotic expression system (E. coli). This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5.
Características del producto
category
Proteins and Peptides
origin
Human
host
E. coli
immunogen target
Lys-63-Specific Deubiquitinase BRCC36 (BRCC3)
host
E. coli
conjugation
Unconjugated
form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
tested applications
WB, SDS-PAGE
size 1
10 µg
size 2
50 µg
size 3
100 µg
size 4
200 µg
size 5
500 µg
observerd MW
Molecular Weight: Calculated MW: 19.8 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Gln7-Gly148 Tag: N-terminal His tag
expression
Recombinant
purity
> 95%
buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
or code
BRCC3
dry ice
No
availability
Shipped within 5-7 working days.
uniprot id
NCBI accession
NP_001018065.1, NM_001018055.2, NP_001229569.1, NM_001242640.1, NP_077308.1, NM_024332.3
note
This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
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proveedor
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