Human Lys-63-Specific Deubiquitinase BRCC36 (BRCC3) Protein

Human Lys-63-Specific Deubiquitinase BRCC36 (BRCC3) Protein

Pida mas información

935106861

info@markelab.com

Precio

208€ (10 µg)

Protein BRCC3

proveedor

Abbexa

reference

abx650811

Tested Applications

WB, SDS-PAGE

reactivity

status

RUO

clonality

Descripción

Human Lys-63-Specific Deubiquitinase BRCC36 (BRCC3) Protein is a recombinant Human protein produced in a Prokaryotic expression system (E. coli). This gene encodes a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. This complex plays a role in the DNA damage response, where it is responsible for the stable accumulation of BRCA1 at DNA break sites. The component encoded by this gene can specifically cleave Lys 63-linked polyubiquitin chains, and it regulates the abundance of these polyubiquitin chains in chromatin. The loss of this gene results in abnormal angiogenesis and is associated with syndromic moyamoya, a cerebrovascular angiopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 5.

Características del producto

category

Proteins and Peptides


origin

Human


host

E. coli


immunogen target

Lys-63-Specific Deubiquitinase BRCC36 (BRCC3)


host

E. coli


conjugation

Unconjugated


form

Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.


tested applications

WB, SDS-PAGE


size 1

10 µg


size 2

50 µg


size 3

100 µg


size 4

200 µg


size 5

500 µg


observerd MW

Molecular Weight: Calculated MW: 19.8 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Gln7-Gly148 Tag: N-terminal His tag


expression

Recombinant


purity

> 95%


buffer

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.


storage

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.


or code

BRCC3


dry ice

No


availability

Shipped within 5-7 working days.


NCBI accession

NP_001018065.1, NM_001018055.2, NP_001229569.1, NM_001242640.1, NP_077308.1, NM_024332.3


note

This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.


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