Human Keratin, Type I Cytoskeletal 18 (KRT18) Protein

category

Proteins and Peptides

origin

Human

host

E. coli

immunogen target

Keratin, Type I Cytoskeletal 18 (KRT18)

host

E. coli

conjugation

Unconjugated

form

Lyophilized Reconstitute in sterile 18 MΩ · cm water to a concentration of at least 0.7 mg/ml. This stock solution can be further diluted if required. Reconstitution to filaments: Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS.

tested applications

SDS-PAGE

size 1

5 µg

size 2

20 µg

size 3

1 mg

observerd MW

Molecular Weight: Calculated MW: 48.2 kDa Observed MW (SDS-PAGE): 45 kDa Concentration: Prior to lyophilization: 1 mg/ml

expression

Recombinant

purity

> 95% (SDS-PAGE)

purification

Purified by proprietary chromatographic techniques.

buffer

Prior to lyophilization: 30 mM Tris-HCl, pH 8, containing 9.5 M urea, 2 mM EDTA, 2 mM DTT and 1 0mM methylammonium chloride.

storage

Stable at room temperature for up to 3 weeks. Store below -18 °C for long-term storage. Upon reconstitution, store at 4 °C for up to 7 days and store below -18 °C for long-term storage. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze/thaw cycles.

or code

KRT18

dry ice

No

availability

Shipped within 5-10 working days.

uniprot id

note

This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.