Human Keratin, Type I Cytoskeletal 18 (KRT18) Protein

Proteins and Peptides

Keratin, Type I Cytoskeletal 18 (KRT18)

E. coli

Human

Unconjugated

Molecular Weight: Calculated MW: 48.2 kDa  Observed MW (SDS-PAGE): 45 kDa

Concentration: Prior to lyophilization: 1 mg/ml

Recombinant

> 95% (SDS-PAGE)

Purified by proprietary chromatographic techniques.

5 µg

20 µg

1 mg

Lyophilized Reconstitute in sterile 18 MΩ · cm water to a concentration of at least 0.7 mg/ml. This stock solution can be further diluted if required.  Reconstitution to filaments:  Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS.

SDS-PAGE

Prior to lyophilization: 30 mM Tris-HCl, pH 8, containing 9.5 M urea, 2 mM EDTA, 2 mM DTT and 1 0mM methylammonium chloride.

Shipped within 5-10 working days.

Stable at room temperature for up to 3 weeks. Store below -18 °C for long-term storage. Upon reconstitution, store at 4 °C for up to 7 days and store below -18 °C for long-term storage. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze/thaw cycles.

No

P05783

Protein KRT18

RUO

This product is for research use only.   Not for human consumption, cosmetic, therapeutic or diagnostic use.