Human Hepcidin (HAMP) Protein

Proteins and Peptides

Hepcidin (HAMP)

293F cells

Activity: Active
Biological Activity: Hepcidin is a regulator of iron metabolism, inhibiting iron transport by binding to the iron export channel Ferroportin (FPN) which is located on the basolateral surface of gut enterocytes and the plasma membrane of reticuloendothelial cells (macrophages). Hepcidin ultimately breaks down the transporter protein in the lysosome. Inhibiting FPN prevents iron from being exported, resulting in iron being sequestered in the cells. A binding ELISA assay was conducted to detect the interaction of recombinant human Hepcidin and recombinant human FPN. Briefly, Hepcidin was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FPN-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-Hepcidin polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. 
Sequence Fragment: Ser25-Thr84
Tag: N-terminal His Tag and Fc tag

Human

Unconjugated

Calculated MW: 34.1 kDa  Observed MW (SDS-PAGE): 34 kDa, 38 kDa

Recombinant

> 80%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized

WB, SDS-PAGE

Prior to lyophilization: PBS, pH 7.4, containing 5% Trehalose.

Shipped within 5-7 working days.

Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.

No

P81172

57817

HEPC,PLTR,HFE2B,LEAP1,Putative liver tumor regressor,PLTR

Protein HAMP

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Endotoxin Level: < 1.0 EU/µg (LAL method)
Concentration: Prior to lyophilization: 400 µg/ml