Human Hepatocyte Growth Factor (HGF) Protein

category

Proteins and Peptides

origin

Human

host

E. coli

immunogen target

Hepatocyte Growth Factor (HGF)

host

E. coli

conjugation

Unconjugated

form

Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.

tested applications

WB, SDS-PAGE

size 1

10 µg

size 2

50 µg

size 3

100 µg

size 4

200 µg

size 5

500 µg

observerd MW

Molecular Weight: Calculated MW: 58.5 kDa Observed MW (SDS-PAGE): 70 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Gln32-Asn291 Tag: N-terminal His tag and GST tag

expression

Recombinant

purity

> 90%

buffer

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose.

storage

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.

or code

HGF

dry ice

No

availability

Shipped within 5-12 working days.

alias

SF,HGFB,HPTA,F-TCF,DFNB39,Fibroblast-Derived Tumor Cytotoxic Factor

uniprot id

note

This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.