Human Growth Factor Receptor Bound Protein 2 (Grb2) Protein

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935106861
info@markelab.com
Precio
455€ (50 µg)
Protein GRB2
proveedor
Abbexareference
abx651493Tested Applications
WB, SDS-PAGEreactivity
status
RUOclonality
Descripción
Growth Factor Receptor Bound Protein 2 (Grb2) Protein is a Human protein produced in 293F cell.
Background
GRB2 is an adaptor protein that links receptor tyrosine kinases (RTKs) to downstream signaling pathways, including Ras/MAPK and PI3K/Akt. It consists of one SH2 domain flanked by two SH3 domains, enabling it to interact with phosphorylated tyrosines and proline-rich regions of target proteins. GRB2 primarily binds to proteins like SOS (a Ras GEF), Shc, and PLCγ, facilitating Ras activation and subsequent signaling for cell growth, proliferation, and differentiation. GRB2 is essential for embryonic development, cellular homeostasis, and immune responses. Dysregulation of GRB2 contributes to oncogenesis, particularly in breast, lung, and gastric cancers, where it enhances cell proliferation and survival through constitutive Ras signaling. GRB2 also plays roles in immune cell signaling, particularly T-cell receptor activation and cytokine production. Knockout models demonstrate embryonic lethality, highlighting its critical function in cellular signaling and development.
Características del producto
category
Proteins and Peptides
origin
Human
host
293F cell
immunogen target
Growth Factor Receptor Bound Protein 2 (Grb2)
host
293F cell
conjugation
Unconjugated
form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
tested applications
WB, SDS-PAGE
size 1
50 µg
size 2
100 µg
size 3
200 µg
size 4
500 µg
size 5
1 mg
observerd MW
Molecular Weight: Calculated MW: 26.8 kDa Observed MW: 27 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Met1-Val217 Tag: N-terminal His tag
expression
Recombinant
purity
> 95%
buffer
Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 5% Trehalose and Proclin-300.
storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
or code
GRB2
dry ice
No
availability
Shipped within 5-7 working days.
alias
ASH, EGFRBP-Grb3-3, MST084, MSTP084, NCKAP2
uniprot id
note
This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
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