Human Growth Factor Receptor Bound Protein 2 (Grb2) Protein

Este producto es parte de GRB - Growth factor receptor bound protein
Human Growth Factor Receptor Bound Protein 2 (Grb2) Protein

Pida mas información

935106861

info@markelab.com

Precio

234€ (10 µg)

Protein GRB2

proveedor

Abbexa

reference

abx066953

Tested Applications

WB, SDS-PAGE

reactivity

status

RUO

clonality

Descripción

Recombinant Growth Factor Receptor Bound Protein 2 (GRB2) is a recombinant Human protein produced in a Prokaryotic expression system (E. coli).


Background

GRB2 is an adaptor protein that links receptor tyrosine kinases (RTKs) to downstream signaling pathways, including Ras/MAPK and PI3K/Akt. It consists of one SH2 domain flanked by two SH3 domains, enabling it to interact with phosphorylated tyrosines and proline-rich regions of target proteins. GRB2 primarily binds to proteins like SOS (a Ras GEF), Shc, and PLCγ, facilitating Ras activation and subsequent signaling for cell growth, proliferation, and differentiation. GRB2 is essential for embryonic development, cellular homeostasis, and immune responses. Dysregulation of GRB2 contributes to oncogenesis, particularly in breast, lung, and gastric cancers, where it enhances cell proliferation and survival through constitutive Ras signaling. GRB2 also plays roles in immune cell signaling, particularly T-cell receptor activation and cytokine production. Knockout models demonstrate embryonic lethality, highlighting its critical function in cellular signaling and development.

Características del producto

category

Proteins and Peptides


origin

Human


host

E. coli


immunogen target

Growth Factor Receptor Bound Protein 2 (Grb2)


host

E. coli


conjugation

Unconjugated


form

Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.


tested applications

WB, SDS-PAGE


size 1

10 µg


size 2

50 µg


size 3

100 µg


size 4

200 µg


size 5

500 µg


observerd MW

Molecular Weight: Calculated MW: 29.1 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Met1-Val217 Tag: N-terminal His tag


expression

Recombinant


purity

> 95%


buffer

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.


storage

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.


or code

GRB2


dry ice

No


availability

Shipped within 5-7 working days.


alias

ASH, EGFRBP-Grb3-3, MST084, MSTP084, NCKAP2


note

This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.


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