Human Diphthine synthase (DPH5) ELISA Kit
715€ (96 tests)
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935106861
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name
Human Diphthine synthase (DPH5) ELISA Kit
category
ELISA Kits
provider
Abbexa
reference
abx510890
tested applications
ELISA
Description
Human Diphthine synthase (DPH5) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Human Diphthine synthase (DPH5) concentrations in serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids.
Documents del producto
Instrucciones
Data sheet
Product specifications
| Category | ELISA Kits |
| Immunogen Target | Diphthine synthase (DPH5) |
| Reactivity | Human |
| Detection Method | Colorimetric |
| Assay Data | Quantitative |
| Test Range | 0.156 ng/ml - 10 ng/ml |
| Recommended Dilution | Optimal dilutions/concentrations should be determined by the end user. |
| Size 1 | 96 tests |
| Form | Lyophilized |
| Tested Applications | ELISA |
| Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids. |
| Availability | Shipped within 5-15 working days. The validity for this kit is 6 months. |
| Storage | Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual. |
| Dry Ice | No |
| UniProt ID | Q9H2P9 |
| Alias | AD-018,CGI-30,NEDSFF,NPD015,HSPC143,Diphthamide biosynthesis methyltransferase,Diphthine methyl ester synthase |
| Background | Elisa kits for DPH5 |
| Status | RUO |
| Note | Validity: The validity for this kit is 6 months. This product is for research use only. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein. |
Descripción
DPH5 is a methyltransferase enzyme involved in the second step of diphthamide biosynthesis, facilitating the trimethylation of the amino-carboxypropyl intermediate generated by the DPH1-DPH2 complex. This modification is critical for the full maturation of diphthamide on eukaryotic elongation factor 2 (eEF2), which is required for ribosomal translocation during protein synthesis. Mutations in DPH5 disrupt the trimethylation process, leading to incomplete diphthamide synthesis and cellular resistance to diphtheria toxin. Structural studies of DPH5 have revealed highly conserved S-adenosylmethionine (SAM)-binding domains that enable its catalytic function. DPH5 is localized in the cytoplasm, where it interacts with downstream components of the diphthamide biosynthetic pathway. Expression analyses show that DPH5 is ubiquitously present in eukaryotic cells, but its levels are particularly high in tissues with active protein translation. Functional deficiencies in DPH5 result in translational inefficiencies, reduced cellular growth, and increased susceptibility to stress. Its evolutionary conservation and indispensable role in protein synthesis highlight DPH5's importance across eukaryotic organisms.
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