Human Dedicator Of Cytokinesis 1 (DOCK1) Protein
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935106861
info@markelab.com
Precio
221€ (10 µg)
Protein DOCK1
proveedor
Abbexareference
abx066282Tested Applications
WB, SDS-PAGEreactivity
status
RUOclonality
Descripción
Recombinant Dedicator Of Cytokinesis 1 (DOCK1) is a recombinant Human protein produced in a Prokaryotic expression system (E. coli).
Background
DOCK1, also known as DOCK180, is a GEF that activates Rac1 GTPase, regulating cytoskeleton rearrangement and cell migration. It forms a DOCK1–ELMO–Crk complex for Rac1 activation, driving lamellipodia formation and cell motility. DOCK1 is crucial for phagocytosis, cell polarity, neural crest migration, and cardiovascular development. Overexpression links to cancer metastasis by promoting tumor motility. It localizes to cytoplasm and membrane ruffles during migration and is regulated via phosphorylation and scaffold interactions. Knockout studies in mice reveal its role in embryonic viability, neuronal differentiation, and immune responses, highlighting its importance in cell adhesion and morphology.
Características del producto
category
Proteins and Peptides
origin
Human
host
E. coli
immunogen target
Dedicator Of Cytokinesis 1 (DOCK1)
host
E. coli
conjugation
Unconjugated
form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
tested applications
WB, SDS-PAGE
size 1
10 µg
size 2
50 µg
size 3
100 µg
size 4
200 µg
size 5
500 µg
observerd MW
Molecular Weight: Calculated MW: 31.9 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Thr1200-Gln1435 Tag: N-terminal His tag
expression
Recombinant
purity
> 95%
buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
or code
DOCK1
dry ice
No
availability
Shipped within 5-7 working days.
alias
DOCK1, DOCK180, ced5, Dock180, dedicator of cytokinesis 1
uniprot id
note
This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
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