Human Dedicator Of Cytokinesis 1 (DOCK1) Protein

Este producto es parte de DOCK - Dedicator of Cytokinesis
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221€ (10 µg)

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935106861
info@markelab.com
name
Human Dedicator Of Cytokinesis 1 (DOCK1) Protein
category
Proteins and Peptides
provider
Abbexa
reference
abx066282
tested applications
WB, SDS-PAGE

Description

Recombinant Dedicator Of Cytokinesis 1 (DOCK1) is a recombinant Human protein produced in a Prokaryotic expression system (E. coli).

Documents del producto

Instrucciones
Data sheet
Descargar

Product specifications

Category
Proteins and Peptides
Immunogen Target
Dedicator Of Cytokinesis 1 (DOCK1)
Host
E. coli
Origin
Human
Conjugation
Unconjugated
Observed MW
Molecular Weight: Calculated MW: 31.9 kDa
Concentration: Prior to lyophilization: 200 µg/ml
Sequence Fragment: Thr1200-Gln1435
Tag: N-terminal His tag
Expression
Recombinant
Purity
> 95%
Size 1
10 µg
Size 2
50 µg
Size 3
100 µg
Size 4
200 µg
Size 5
500 µg
Form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Tested Applications
WB, SDS-PAGE
Buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
Availability
Shipped within 5-7 working days.
Storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
Dry Ice
No
UniProt ID
Q14185
Alias
DOCK1, DOCK180, ced5, Dock180, dedicator of cytokinesis 1
Background
Protein DOCK1
Status
RUO
Note
This product is for research use only.   Not for human consumption, cosmetic, therapeutic or diagnostic use.

Descripción

DOCK1, also known as DOCK180, is a GEF that activates Rac1 GTPase, regulating cytoskeleton rearrangement and cell migration. It forms a DOCK1–ELMO–Crk complex for Rac1 activation, driving lamellipodia formation and cell motility. DOCK1 is crucial for phagocytosis, cell polarity, neural crest migration, and cardiovascular development. Overexpression links to cancer metastasis by promoting tumor motility. It localizes to cytoplasm and membrane ruffles during migration and is regulated via phosphorylation and scaffold interactions. Knockout studies in mice reveal its role in embryonic viability, neuronal differentiation, and immune responses, highlighting its importance in cell adhesion and morphology.

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