Human Dedicator Of Cytokinesis 1 (DOCK1) Protein

221€ (10 µg)
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935106861
info@markelab.com
name
Human Dedicator Of Cytokinesis 1 (DOCK1) Protein
category
Proteins and Peptides
provider
Abbexa
reference
abx066282
tested applications
WB, SDS-PAGE
Description
Recombinant Dedicator Of Cytokinesis 1 (DOCK1) is a recombinant Human protein produced in a Prokaryotic expression system (E. coli).
Documents del producto
Instrucciones
Data sheet
Product specifications
Category | Proteins and Peptides |
Immunogen Target | Dedicator Of Cytokinesis 1 (DOCK1) |
Host | E. coli |
Origin | Human |
Conjugation | Unconjugated |
Observed MW | Molecular Weight: Calculated MW: 31.9 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Thr1200-Gln1435 Tag: N-terminal His tag |
Expression | Recombinant |
Purity | > 95% |
Size 1 | 10 µg |
Size 2 | 50 µg |
Size 3 | 100 µg |
Size 4 | 200 µg |
Size 5 | 500 µg |
Form | Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
Tested Applications | WB, SDS-PAGE |
Buffer | Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300. |
Availability | Shipped within 5-7 working days. |
Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
Dry Ice | No |
UniProt ID | Q14185 |
Alias | DOCK1, DOCK180, ced5, Dock180, dedicator of cytokinesis 1 |
Background | Protein DOCK1 |
Status | RUO |
Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |
Descripción
DOCK1, also known as DOCK180, is a GEF that activates Rac1 GTPase, regulating cytoskeleton rearrangement and cell migration. It forms a DOCK1–ELMO–Crk complex for Rac1 activation, driving lamellipodia formation and cell motility. DOCK1 is crucial for phagocytosis, cell polarity, neural crest migration, and cardiovascular development. Overexpression links to cancer metastasis by promoting tumor motility. It localizes to cytoplasm and membrane ruffles during migration and is regulated via phosphorylation and scaffold interactions. Knockout studies in mice reveal its role in embryonic viability, neuronal differentiation, and immune responses, highlighting its importance in cell adhesion and morphology.
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