Human Collagen Type IV Alpha 3 (COL4A3) Protein

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935106861
info@markelab.com
Precio
221€ (10 µg)
Protein COL4A3
proveedor
Abbexareference
abx168068Tested Applications
WB, SDS-PAGEreactivity
status
RUOclonality
Descripción
Human COL4A3 Protein is a recombinant Human protein expressed in E. coli.
Background
COL4A3 is one of the six α-chains that compose type IV collagen, a major structural component of basement membranes. COL4A3 assembles into a triple-helical network with COL4A4 and COL4A5 to provide mechanical support, structural stability, and selective permeability in tissues such as the kidney glomerulus, lung alveoli, and the ocular lens. Mutations in COL4A3 are associated with Alport syndrome, a hereditary disorder characterized by progressive kidney disease, sensorineural hearing loss, and ocular abnormalities caused by defective basement membranes. It also plays a role in tissue repair and angiogenesis by interacting with signaling molecules such as integrins and matrix metalloproteinases (MMPs). Dysregulated COL4A3 expression contributes to fibrosis and tumor metastasis by influencing extracellular matrix (ECM) remodeling. Knockout models show abnormal glomerular basement membranes, hematuria, and progressive renal failure, highlighting COL4A3's critical role in basement membrane assembly, tissue integrity, and kidney function.
Características del producto
category
Proteins and Peptides
origin
Human
host
E. coli
immunogen target
Collagen Type IV Alpha 3 (COL4A3)
host
E. coli
conjugation
Unconjugated
form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
tested applications
WB, SDS-PAGE
size 1
10 µg
size 2
50 µg
size 3
100 µg
size 4
200 µg
size 5
500 µg
observerd MW
Molecular Weight: Calculated MW: 30.5 kDa Observed MW (SDS-PAGE): 30 kDa Concentration: Prior to lyophilization: 500 µg/ml Sequence Fragment: Leu1428-His1670 Tag: N-terminal His tag
expression
Recombinant
purity
> 97%
buffer
Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose.
storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
or code
COL4A3
dry ice
No
availability
Shipped within 5-7 working days.
alias
ATS2,ATS3,BFH2,ATS3A,ATS3B,Goodpasture antigen
uniprot id
note
This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
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