Human Carboxylesterase 1 (CES1) Protein

Proteins and Peptides

Carboxylesterase 1 (CES1)

E. coli

Activity: Active
Biological Activity: Carboxylesterase 1 (CES1), also known as Liver Carboxylesterase 1, is a serine esterase and member of a large multigene carboxylesterase family. The protein is involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. It hydrolyses aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA esters. It hydrolyses the methyl ester group of cocaine to form benzoylecgonine. Therefore, the recombinant human CES1 activity was measured by its ability to hydrolyze 4-nitrophenyl acetate (4-NPA) to 4-nitrophenol. The reaction was performed in 50 mM Tris, pH 7.5, initiated by the addition 50 µl of various concentrations of CES1 to 50 µl of 2 mM 4-NPA (100 mM stock in Acetone, diluted with distilled water). The solutions were incubated at 37 °C for 10 min, and then the absorbance was read at 400 nm.
The specific activity was determined to be 1396 U. One unit of enzyme activity is defined as 1 µg of enzyme required to convert 1 pmol of 4-nitrophenyl acetate to 4-nitrophenol in 1 min at 37 °C.
Sequence Fragment: Gly18-Leu299
Tag: N-terminal His tag

Human

Unconjugated

Calculated MW: 34.0 kDa  Observed MW (SDS-PAGE): 35 kDa

Recombinant

> 90%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized

WB, SDS-PAGE

Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.05% Sarcosyl and 5% trehalose.

Shipped within 5-7 working days.

Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.

No

P23141

Protein CES1

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Concentration: Prior to lyophilization: 200 µg/ml