Human Breast Cancer Susceptibility Protein 2 (BRCA2) Protein
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Description
Human Breast Cancer Susceptibility Protein 2 (BRCA2) is a recombinant Human protein produced in a Prokaryotic expression system (E. coli).
Documents del producto
Product specifications
| Category | Proteins and Peptides |
| Immunogen Target | Breast Cancer Susceptibility Protein 2 (BRCA2) |
| Host | E. coli |
| Origin | Human |
| Conjugation | Unconjugated |
| Observed MW | Molecular Weight: Calculated MW: 29.9 kDa Observed MW (SDS-PAGE): 32 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Lys2308-His2537 Tag: N-terminal His tag |
| Expression | Recombinant |
| Purity | > 95% |
| Size 1 | 10 µg |
| Size 2 | 50 µg |
| Size 3 | 100 µg |
| Size 4 | 200 µg |
| Size 5 | 500 µg |
| Form | Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
| Tested Applications | WB, SDS-PAGE |
| Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300. |
| Availability | Shipped within 5-7 working days. |
| Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| UniProt ID | P51587 |
| Alias | BRCC2,BROVCA2,FACD, FAD,FAD1,FANCD,FANCD1,GLM3,PNCA2,XRCC11,Fanconi anemia group D1 protein |
| Background | Protein BRCA2 |
| Status | RUO |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |
Descripción
BRCA2 is a tumor suppressor protein that plays a pivotal role in maintaining genomic stability by facilitating the repair of DNA double-strand breaks through homologous recombination. It interacts with RAD51, a recombinase protein, to mediate the precise alignment and strand exchange during DNA repair. Structurally, BRCA2 contains eight BRC repeats that directly bind RAD51 and a DNA-binding domain that targets damaged DNA regions. Mutations in the BRCA2 gene are strongly associated with an increased risk of hereditary breast, ovarian, and other cancers, such as pancreatic and prostate cancer. These mutations often lead to truncated or dysfunctional proteins, resulting in impaired DNA repair and genomic instability, which drives tumorigenesis. Beyond its role in DNA repair, BRCA2 is also implicated in cell cycle regulation and replication fork protection. BRCA2 deficiency is therapeutically targeted using PARP inhibitors, exploiting synthetic lethality to selectively kill BRCA2-mutant cancer cells while sparing normal cells. This approach has revolutionized treatment options for BRCA2-associated cancers, emphasizing its clinical significance.
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