Human BH3 Interacting Domain Death Agonist (Bid) Protein
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935106861
info@markelab.com
Precio
195€ (10 µg)
Protein BID
proveedor
Abbexareference
abx065536Tested Applications
WB, SDS-PAGEreactivity
status
RUOclonality
Descripción
Recombinant BH3 Interacting Domain Death Agonist (Bid) is a recombinant Human protein produced in a Prokaryotic expression system (E. coli).
Background
BH3-interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl-2 family that plays a crucial role in the mitochondrial pathway of apoptosis BID contains a BH3 domain that enables it to interact with anti-apoptotic Bcl-2 family members, promoting apoptosis in response to various stress signals When activated, BID undergoes proteolytic cleavage, resulting in the generation of a truncated form, tBID, which translocates to the mitochondria and triggers the release of cytochrome c from the mitochondrial membrane This release activates the caspase cascade, leading to cell death BID is critical in the cellular response to DNA damage, oxidative stress, and death receptor signaling It also plays a role in immune cell homeostasis, particularly in regulating the survival of T-cells and the elimination of damaged or infected cells BID’s involvement in apoptosis has made it a significant focus in cancer research, where its activity can be modulated to promote tumor cell death Additionally, BID’s role in maintaining cellular integrity under stress underscores its importance in preventing disease progression and maintaining healthy tissue turnover
Características del producto
category
Proteins and Peptides
origin
Human
host
E. coli
immunogen target
BH3 Interacting Domain Death Agonist (Bid)
host
E. coli
conjugation
Unconjugated
form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
tested applications
WB, SDS-PAGE
size 1
10 µg
size 2
50 µg
size 3
100 µg
size 4
200 µg
size 5
500 µg
observerd MW
Molecular Weight: Calculated MW: 25.8 kDa Observed MW (SDS-PAGE): 27 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Met1-Asp195 Tag: N-terminal His tag
expression
Recombinant
purity
> 95%
buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
or code
BID
dry ice
No
availability
Shipped within 5-7 working days.
alias
p22 BID,FP497
uniprot id
note
This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
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