Human Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B) Protein
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935106861
info@markelab.com
Precio
208€ (10 µg)
Protein ALOX12B
proveedor
Abbexareference
abx650287Tested Applications
WB, SDS-PAGEreactivity
status
RUOclonality
Descripción
Human ALOX12B Protein is a recombinant Human protein produced in a Prokaryotic expression system (E. coli).
Background
ALOX12B is a lipoxygenase enzyme that specifically oxygenates arachidonic acid to form 12(R)-hydroperoxyeicosatetraenoic acid [12(R)-HPETE], which is further metabolized to 12(R)-HETE. ALOX12B is primarily expressed in the epidermis, where it plays a critical role in skin barrier formation and maintenance. It is involved in the biosynthesis of specialized lipids required for the development of the cornified lipid envelope, a structure essential for skin permeability and protection. Mutations in ALOX12B are linked to autosomal recessive congenital ichthyosis (ARCI), a skin disorder characterized by scaling, dryness, and impaired barrier function due to defective lipid metabolism. Beyond its role in skin biology, ALOX12B has been implicated in inflammation and wound healing processes. Dysregulation of ALOX12B may contribute to skin-related disorders and inflammatory skin diseases, making it an important target for understanding epidermal homeostasis and barrier repair therapies.
Características del producto
category
Proteins and Peptides
origin
Human
host
E. coli
immunogen target
Arachidonate-12-Lipoxygenase, 12R Type (ALOX12B)
host
E. coli
conjugation
Unconjugated
form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
tested applications
WB, SDS-PAGE
size 1
10 µg
size 2
50 µg
size 3
100 µg
size 4
200 µg
size 5
500 µg
observerd MW
Molecular Weight: Calculated MW: 29.6 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Leu479-Ile701 Tag: N-terminal His tag
expression
Recombinant
purity
> 90%
buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
or code
ALOX12B
dry ice
No
availability
Shipped within 5-7 working days.
alias
12R-LOX,12R-lipoxygenase,Epidermis-type lipoxygenase 12,arachidonate lipoxygenase 3
uniprot id
note
This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
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