Human Alanine Glyoxylate Aminotransferase (AGXT) Protein

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208€ (10 µg)

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935106861
info@markelab.com
name
Human Alanine Glyoxylate Aminotransferase (AGXT) Protein
category
Proteins and Peptides
provider
Abbexa
reference
abx168878
tested applications
WB, SDS-PAGE

Description

Human AGXT Protein is a recombinant Human protein produced in a Prokaryotic expression system (E. coli).

Documents del producto

Instrucciones
Data sheet
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Product specifications

Category
Proteins and Peptides
Immunogen Target
Alanine Glyoxylate Aminotransferase (AGXT)
Host
E. coli
Origin
Human
Conjugation
Unconjugated
Observed MW
Molecular Weight: Calculated MW: 23.8 kDa  Observed MW: 24 kDa

Concentration: Prior to lyophilization: 120 µg/ml

Sequence Fragment: Gly63-Leu247

Tag: N-terminal His tag
Expression
Recombinant
Purity
> 90%
Size 1
10 µg
Size 2
50 µg
Size 3
100 µg
Size 4
200 µg
Size 5
500 µg
Form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Tested Applications
WB, SDS-PAGE
Buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose.
Availability
Shipped within 5-7 working days.
Storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
Dry Ice
No
UniProt ID
P21549
Gene ID
189
Alias
AGXT, AGT1, SPAT
Background
Protein AGXT
Status
RUO
Note
This product is for research use only.   Not for human consumption, cosmetic, therapeutic or diagnostic use.

Descripción

Alanine-glyoxylate aminotransferase (AGXT) is a pyridoxal phosphate-dependent enzyme localized in peroxisomes, where it plays a critical role in detoxifying glyoxylate by converting it into glycine, using alanine as an amino donor. AGXT is central to preventing the overproduction of oxalate, a byproduct of glyoxylate metabolism, which, if accumulated, can form calcium oxalate crystals leading to kidney stones and renal damage. Mutations in the AGXT gene cause primary hyperoxaluria type 1 (PH1), a rare autosomal recessive disorder characterized by excessive oxalate production, kidney stone formation, and systemic oxalosis. AGXT's activity is regulated by intracellular localization and metabolic cues, and therapies targeting AGXT include enzyme replacement therapy and gene therapy to restore glyoxylate metabolism in affected individuals.

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