Human Adenosine Deaminase (ADA) Protein

Este producto es parte de ADA - Adenosine Deaminase
Human Adenosine Deaminase (ADA) Protein
221€ (10 µg)

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Name
Human Adenosine Deaminase (ADA) Protein
Category
Proteins and Peptides
Provider
Abbexa
Reference
abx065150
Tested Applications
WB, SDS-PAGE

Description

Human ADA (ADA) is a recombinant Human protein expressed in E. coli.

Documentos del producto

Instrucciones
Data sheet
Descargar

Especificaciones del producto

Category
Proteins and Peptides
Immunogen Target
Adenosine Deaminase (ADA)
Host
E. coli
Assay Type
Activity: Not tested
Sequence Fragment: Lys11-Val280
Tag: N-terminal His tag
Origin
Human
Conjugation
Unconjugated
Observed MW
Calculated MW: 57.2 kDa
Expression
Recombinant
Purity
> 95%
Size 1
10 µg
Size 2
50 µg
Size 3
100 µg
Size 4
200 µg
Size 5
500 µg
Form
Lyophilized
Tested Applications
WB, SDS-PAGE
Buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
Availability
Shipped within 5-7 working days.
Storage
Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.
Dry Ice
No
UniProt ID
P00813
Alias
Adenosine aminohydrolase
Background
Protein ADA
Status
RUO
Note
THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Concentration: Prior to lyophilization: 200 µg/ml

Background

Adenosine deaminase (ADA) is an enzyme crucial in purine metabolism, responsible for catalyzing the irreversible deamination of adenosine and deoxyadenosine to inosine and deoxyinosine, respectively. ADA is predominantly found in lymphocytes, with high activity in tissues such as the thymus, spleen, and bone marrow. It is vital for the development and maintenance of the immune system, as its deficiency leads to the accumulation of toxic purine metabolites, which impair DNA synthesis and lymphocyte survival. Mutations in the ADA gene result in severe combined immunodeficiency (SCID), characterized by a lack of functional T, B, and NK cells. ADA is also widely studied as a marker of immune activation and inflammation in conditions like tuberculosis and rheumatoid arthritis. Therapeutic interventions for ADA deficiency include enzyme replacement therapy and gene therapy, both of which aim to restore immune function.

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