Glutamate Decarboxylase 1 (GAD1) Antibody

Glutamate Decarboxylase 1 (GAD1) Antibody
195€ (20 µl)

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Name
Glutamate Decarboxylase 1 (GAD1) Antibody
Category
Primary Antibodies
Provider
Abbexa
Reference
abx002144
Tested Applications
ELISA, WB, IHC, IF/ICC

Description

Glutamate Decarboxylase 1 (GAD1) Antibody is a Rabbit Polyclonal antibody against Glutamate Decarboxylase 1 (GAD1). This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

Documentos del producto

Instrucciones
Data sheet
Descargar

Especificaciones del producto

Category
Primary Antibodies
Immunogen Target
Target: Glutamate Decarboxylase 1 (GAD1)
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human GAD67/GAD67/GAD1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Assay Type
Concentration: > 0.2 mg/ml
Recommended Dilution
ELISA: 1 µg/ml, WB: 1/500 - 1/1000, IHC-P: 1/50 - 1/200, IF/ICC: 1/50 - 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Clonality
Polyclonal
Conjugation
Unconjugated
Isotype
IgG
Observed MW
Calculated MW: 67 kDa  Observed MW: 67 kDa
Purification
Purified by affinity chromatography.
Size 1
20 µl
Size 2
100 µl
Size 3
2 × 100 µl
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF/ICC
Buffer
PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol.
Availability
Shipped within 5-10 working days.
Storage
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Dry Ice
No
UniProt ID
Q99259, P48318, P18088
Gene ID
2571, 14415
NCBI Accession
NP_000808.2
OMIM
603513
Background
Antibody anti-GAD1
Status
RUO
Note
THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.

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