FastPfu DNA Polymerase Enzyme (with 2.5 mM dNTPs)

Proteins and Peptides

FastPfu DNA Polymerase

Unconjugated

250 U

500 U

3 kU

PCR

FastPfu DNA Polymerase: Contains 50 mM Tris-HCl (pH 8.2), 0.1 mM EDTA, 1 mM DTT, Stabilizers, 50% glycerol. <br> FastPfu Buffer: Contains 100 mM Tris-SO4 (pH 9.2), 200 mM KCl, 10 mM MgSO4, 10% glycerol.

Shipped within 10-20 working days.

Store at -20 °C for up to 2 years. Avoid repeated freeze/thaw cycles.

No

Protein FastPfu DNA Polymerase

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Reaction Components:    Component  Volume  Final Concentration    Template  Variable  as required    Forward Primer (10 µM)  1 µl  0.2 µM   Reverse Primer (10 µM)  1 µl  0.2 µM 5X FastPfu Buffer 10 µl  1X2.5 mM dNTPs 4 µl  0.2 mM FastPfu DNA Polymerase 1 µl  2.5 U  Nuclease-Free ddH2O  Variable N/A  Total Volume  50 µl N/A    Suggested Reaction Conditions:     Template  Quantity Genomic DNA10-100 ng Genomic DNA Plasmid DNA 1-30 ng Plasmid DNA cDNA 1-2 µl cDNA from RT reaction  (50-500 ng RNA for RT reaction)   Thermal Cycling Conditions:     Number of Cycles  Temperature  Time  1 cycle 95 °C  2 min   30-35 cycles  95 °C 20 seconds TM - 5 °C 20 seconds 72 °C 6 kb/min 1 cycle 72 °C5 min  Notes: The PCR Stimulant can be used to optimize the amplification of complex templates or high GC/AT templates. The recommended working concentration is 0.5X - 2X (concentration of the provided stock solution is 5X). It is recommended to add the FastPfu DNA Polymerase last in the reaction system. For GC-rich templates, the recommended denaturation temperature is 98 °C. If precipitates in the FastPfu Buffer are observed, warm to 37 °C in a water bath and mix before use.
Concentration: 2.5 U/µl