EP300 (pS89) Antibody

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292.5€ (80 µl)

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935106861
info@markelab.com
name
EP300 (pS89) Antibody
category
Primary Antibodies
provider
Abbexa
reference
abx031894
tested applications
ELISA, IHC, DB

Description

EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CPB can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.

Documents del producto

Instrucciones
Data sheet
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Product specifications

Category
Primary Antibodies
Immunogen Target
EP300 (pS89)
Host
Rabbit
Reactivity
Human
Assay Data
Modification: Phosphorylation // Target Modification: Ser89
Recommended Dilution
IHC-P: 1/50 - 1/100, DB: 1/500. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Clonality
Polyclonal
Conjugation
Unconjugated
Isotype
IgG
Purification
Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Size 1
80 µl
Size 2
400 µl
Form
Liquid
Tested Applications
ELISA, IHC, DB
Buffer
PBS containing 0.09% sodium azide.
Availability
Shipped within 5-10 working days.
Storage
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Dry Ice
No
UniProt ID
Q09472
NCBI Accession
NP_001420.2
Background
Antibody anti-EP300
Status
RUO

Descripción

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