EP300 (pS89) Antibody

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Description
EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CPB can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.
Documents del producto
Product specifications
Category | Primary Antibodies |
Immunogen Target | EP300 (pS89) |
Host | Rabbit |
Reactivity | Human |
Assay Data | Modification: Phosphorylation // Target Modification: Ser89 |
Recommended Dilution | IHC-P: 1/50 - 1/100, DB: 1/500. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
Clonality | Polyclonal |
Conjugation | Unconjugated |
Isotype | IgG |
Purification | Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
Size 1 | 80 µl |
Size 2 | 400 µl |
Form | Liquid |
Tested Applications | ELISA, IHC, DB |
Buffer | PBS containing 0.09% sodium azide. |
Availability | Shipped within 5-10 working days. |
Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
Dry Ice | No |
UniProt ID | Q09472 |
NCBI Accession | NP_001420.2 |
Background | Antibody anti-EP300 |
Status | RUO |
Descripción
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