E3 Ubiquitin-Protein Ligase CHFR (CHFR) Antibody

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Description
Rabbit polyclonal antibody against CHFR protein. Immunogen region is Internal.
Documents del producto
Product specifications
Category | Primary Antibodies |
Immunogen Target | E3 Ubiquitin-Protein Ligase CHFR (CHFR) |
Host | Rabbit |
Reactivity | Human, Mouse, Rat |
Recommended Dilution | Optimal dilutions/concentrations should be determined by the end user. |
Clonality | Polyclonal |
Conjugation | Unconjugated |
Isotype | IgG |
Purification | Purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. |
Size 1 | 10 µg |
Size 2 | 100 µg |
Size 3 | 200 µg |
Size 4 | 300 µg |
Size 5 | 1 mg |
Form | Liquid |
Tested Applications | WB |
Buffer | PBS (without Mg<sup>2+</sup> and Ca<sup>2+</sup>), pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% glycerol. |
Availability | Shipped within 5-10 working days. |
Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
Dry Ice | No |
UniProt ID | Q96EP1 |
Background | Antibody anti-CHFR |
Status | RUO |
Note | Concentration: 1 mg/ml - |
Descripción
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E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2(UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.
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E3 Ubiquitin-Protein Ligase CHFR (CHFR) Antibody
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