DNA Topoisomerase I, Mitochondrial (TOP1MT) Antibody (FITC)

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Description
DNA Topoisomerase I, Mitochondrial (TOP1MT) Antibody (FITC) is a Rabbit Polyclonal antibody conjugated to FITC for the detection of Human TOP1MT.
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Product specifications
Category | Primary Antibodies |
Immunogen Target | DNA Topoisomerase I, Mitochondrial (TOP1MT) |
Host | Rabbit |
Reactivity | Human |
Recommended Dilution | Optimal dilutions/concentrations should be determined by the end user. |
Clonality | Polyclonal |
Conjugation | FITC |
Isotype | IgG |
Purity | > 95% |
Purification | Purified by Protein G chromatography. |
Size 1 | 20 µg |
Size 2 | 50 µg |
Size 3 | 100 µg |
Size 4 | 200 µg |
Size 5 | 1 mg |
Form | Liquid |
Buffer | 0.01 M PBS, pH 7.4, 0.03% Proclin-300 and 50% glycerol. |
Availability | Shipped within 5-10 working days. |
Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
Dry Ice | No |
UniProt ID | Q969P6 |
Gene ID | 116447 |
OMIM | 606387 |
Background | Antibody anti-TOP1MT |
Status | RUO |
Descripción
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Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone(By similarity).
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