DNA Damage-Binding Protein 1 (DDB1) Antibody
312€ (60 µl)
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Name
DNA Damage-Binding Protein 1 (DDB1) Antibody
Category
Primary Antibodies
Provider
Abbexa
Reference
abx002127
Tested Applications
IHC, IF/ICC
Description
DDB1 Antibody is a Rabbit Polyclonal antibody against DDB1. The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins.
Documentos del producto
Instrucciones
Data sheet
Especificaciones del producto
| Category | Primary Antibodies |
| Immunogen Target | Target: DNA Damage-Binding Protein 1 (DDB1) Immunogen: A synthetic peptide corresponding to human DDB1 |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Assay Type | Concentration: 1 mg/ml |
| Recommended Dilution | IHC-P: 1/50 - 1/100, IF/ICC: 1/50 - 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Purification | Purified by affinity chromatography. |
| Size 1 | 60 µl |
| Size 2 | 120 µl |
| Size 3 | 200 µl |
| Form | Liquid |
| Tested Applications | IHC, IF/ICC |
| Buffer | PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol. |
| Availability | Shipped within 5-10 working days. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| UniProt ID | Q16531 |
| Gene ID | 1642 |
| NCBI Accession | NP_001914.3 |
| Alias | XPE,DDBA,XAP1,XPCE,XPE-BF,UV-DDB1,WHIKERS,UV-damaged DNA-binding factor,DNA damage-binding protein a,DDB p127 subunit |
| Background | Antibody anti-DDB1 |
| Status | RUO |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |
Background
Damage-specific DNA binding protein 1 (DDB1) is a critical component of the ubiquitin-proteasome system, serving as a scaffold protein within the CUL4-DDB1 E3 ubiquitin ligase complex. DDB1 interacts with various substrate receptors, including members of the DCAF (DDB1 and CUL4-associated factors) family, to mediate the recognition and ubiquitination of target proteins. Its role is pivotal in regulating DNA repair, particularly nucleotide excision repair (NER), where it facilitates the removal of UV-induced photoproducts and bulky DNA adducts. DDB1 also contributes to the degradation of proteins involved in transcriptional regulation, cell cycle progression, and genome stability. Structurally, DDB1 contains WD40 domains that mediate interactions with CUL4 and substrate receptors, ensuring versatility and specificity in substrate selection. Dysregulation of DDB1 has been associated with genomic instability and cancer development. Its ubiquitous expression highlights its fundamental role in cellular homeostasis and DNA damage response pathways.