VEGFA antibody

Este producto es parte de VEGFA - Vascular Endothelial Growth Factor A
VEGFA antibody
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Name
VEGFA antibody
Category
Primary Antibodies
Provider
FineTest
Reference
FNab09391
Tested Applications
ELISA, IHC, IF, WB

Description

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1(MVCD1). VEGFA has 13 isoforms with MW 16kd to 45kd. Some isoforms has homodimer form, such as VEGFA189(38kd), VEFGA110(34kd). VEGFA is a 34kd to 42kd, dimeric, disulfide-bound glycoprotein. VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids(PMID:15602010).

Documentos del producto

Instrucciones
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Data sheet
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Especificaciones del producto

Category
Primary Antibodies
Immunogen Target
vascular endothelial growth factor A (VEGFA)
Host
Rabbit
Reactivity
Human, Mouse, Rat
Recommended Dilution
WB: 1:200-1:2000; IF: 1:10-1:100; IHC: 1:50-1:500
Clonality
polyclonal
Conjugation
Unconjugated
Isotype
IgG
Observed MW
15-27kDa, 34-45kDa
Purity
≥95% as determined by SDS-PAGE
Purification
Immunogen affinity purified
Size 1
100µg
Form
liquid
Tested Applications
ELISA, IHC, IF, WB
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
UniProt ID
P15692
Gene ID
7422
Alias
L-VEGF,MVCD1,VEGF,VPF,Vascular permeability factor
Background
Antibody anti-VEGFA
Status
RUO
Note
Mol. Weight 15-27 kDa, 34-45 kDa

Background

VEGFA is a key member of the vascular endothelial growth factor (VEGF) family and plays a crucial role in angiogenesis, vascular permeability, and endothelial cell proliferation VEGFA binds primarily to its receptors VEGFR-1 and VEGFR-2, triggering signaling pathways like PI3K/AKT and ERK/MAPK to promote blood vessel formation during embryonic development, wound healing, and tissue repair Dysregulated VEGFA expression is associated with pathological angiogenesis observed in cancer, diabetic retinopathy, and age-related macular degeneration (AMD) VEGFA also increases vascular permeability by altering endothelial junctions, contributing to fluid leakage in conditions such as inflammation and tumors Targeted therapies, such as bevacizumab and other anti-VEGF agents, inhibit VEGFA activity to suppress angiogenesis and tumor growth in various cancers and ocular diseases

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