RPS3 antibody
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Description
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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Product specifications
| Category | Primary Antibodies |
| Immunogen Target | ribosomal protein S3 (RPS3) |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Recommended Dilution | WB: 1:500 - 1:2000; IHC: 1:50 - 1:200 |
| Clonality | polyclonal |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Observed MW | 27 kDa |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Immunogen affinity purified |
| Size 1 | 100µg |
| Form | liquid |
| Tested Applications | ELISA, WB, IHC |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months (Avoid repeated freeze / thaw cycles.) |
| UniProt ID | P23396 |
| Gene ID | 6188 |
| Alias | Small ribosomal subunit protein uS3,40S ribosomal protein S3,RPS3 |
| Background | Antibody anti-RPS3 |
| Status | RUO |
| Note | Mol. Weight 27 kDa |
Descripción
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RPS3 antibody
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Ver ProductoRPS3 antibody
Involved in translation as a component of the 40S small ribosomal subunit(PubMed:8706699). Has endonuclease activity and plays a role in repair of damaged DNA(PubMed:7775413). Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA(PubMed:15707971). Displays high binding affinity for 7,8-dihydro-8-oxoguanine(8-oxoG), a common DNA lesion caused by reactive oxygen species(ROS)(PubMed:14706345). Has also been shown to bind with similar affinity to intact and damaged DNA(PubMed:18610840). Stimulates the N-glycosylase activity of the base excision protein OGG1(PubMed:15518571). Enhances the uracil excision activity of UNG1(PubMed:18973764). Also stimulates the cleavage of the phosphodiester backbone by APEX1(PubMed:18973764). When located in the mitochondrion, reduces cellular ROS levels and mitochondrial DNA damage(PubMed:23911537). Has also been shown to negatively regulate DNA repair in cells exposed to hydrogen peroxide(PubMed:17049931). Plays a role in regulating transcription as part of the NF-kappa-B p65-p50 complex where it binds to the RELA/p65 subunit, enhances binding of the complex to DNA and promotes transcription of target genes(PubMed:18045535). Represses its own translation by binding to its cognate mRNA(PubMed:20217897). Binds to and protects TP53/p53 from MDM2-mediated ubiquitination(PubMed:19656744). Involved in spindle formation and chromosome movement during mitosis by regulating microtubule polymerization(PubMed:23131551). Involved in induction of apoptosis through its role in activation of CASP8(PubMed:14988002). Induces neuronal apoptosis by interacting with the E2F1 transcription factor and acting synergistically with it to up-regulate pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5(PubMed:20605787). Interacts with TRADD following exposure to UV radiation and induces apoptosis by caspase-dependent JNK activation(PubMed:22510408).
Ver ProductoRPS3 antibody
40S ribosomal protein S3 (RPS3), also named as SW-cl.26, is a 243 amino acid protein,which contains one KH type-2 domain and belongs to the ribosomal protein S3P family. RPS3 localizes in the cytoplasm. RPS3 is identified in a IGF2BP1-dependent mRNP granule complex, which contains untranslated mRNAs. RPS3 plays a role in repairing various DNA damage acting as a repair UV endonuclease. Nuclear accumulation of RPS3 results in an increase in DNA repair activity to some extent, thereby sustaining neuronal survival. The calculated molecular weight of RPS3 is 26 kDa, but the modified RPS3 is about 33 kDa.
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