PHF2 antibody
Por favor contáctenos para obtener información detallada sobre el precio y disponibilidad.
Description
Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3(H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3(H3K4me3) at rDNA promoters and promotes expression of rDNA.
Documents del producto
Product specifications
| Category | Primary Antibodies |
| Immunogen Target | PHD finger protein 2 (PHF2) |
| Host | Rabbit |
| Reactivity | Human |
| Recommended Dilution | WB: 1:200-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200 |
| Clonality | polyclonal |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Observed MW | 100 kDa, 150 kDa |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Immunogen affinity purified |
| Size 1 | 100µg |
| Form | liquid |
| Tested Applications | ELISA, WB, IHC, IP |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| UniProt ID | O75151 |
| Gene ID | 5253 |
| Alias | CENP-35,GRC5, JHDM1E, KDM7C |
| Background | Antibody anti-PHF2 |
| Status | RUO |
| Note | Mol. Weight 100 kDa, 150 kDa |
Descripción
PHF2 is a histone demethylase that specifically targets H3K9me2 to activate transcription by removing repressive methyl marks on histones. It serves as a transcriptional co-activator that regulates gene expression in response to metabolic and cellular stress signals. PHF2 is recruited to gene promoters through interactions with transcription factors and chromatin-associated proteins, promoting the activation of genes involved in lipid metabolism, gluconeogenesis, and inflammatory responses. It is expressed in metabolic tissues such as the liver, skeletal muscle, and adipose tissue, where it plays a role in energy homeostasis and inflammation control. Dysregulation of PHF2 is associated with metabolic diseases, including obesity, diabetes, and cancer, where its role in histone demethylation is disrupted. Knockdown studies reveal decreased transcriptional activation, impaired lipid metabolism, and abnormal inflammatory responses, highlighting its role in epigenetic regulation, metabolism, and cellular stress adaptation.
Related Products
PHF2 antibody
Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3(H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3(H3K4me3) at rDNA promoters and promotes expression of rDNA.
Ver Producto
Human PHD finger protein 2 (PHF2) ELISA Kit
Human PHD finger protein 2 (PHF2) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Human PHF2 concentrations in tissue homogenates, cell lysates and other biological fluids.
Ver Producto