anti- DDB1 antibody
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Precio
Precio a consultar
Antibody anti-DDB1
proveedor
FineTestreference
FNab02285Tested Applications
ELISA, WB, IHC, IFreactivity
human,mouse,ratstatus
RUOclonality
monoclonalDescripción
Required for DNA repair. Binds to DDB2 to form the UV-damaged DNA-binding protein complex(the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway(the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers(CPD), 6-4 photoproducts(6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX(DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2)(also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8)(the CSA complex) plays a role in transcription-coupled repair(TCR). May also play a role in ubiquitination of CDKN1B/p27kip when associated with CUL4 and SKP2.
Background
Damage-specific DNA binding protein 1 (DDB1) is a critical component of the ubiquitin-proteasome system, serving as a scaffold protein within the CUL4-DDB1 E3 ubiquitin ligase complex. DDB1 interacts with various substrate receptors, including members of the DCAF (DDB1 and CUL4-associated factors) family, to mediate the recognition and ubiquitination of target proteins. Its role is pivotal in regulating DNA repair, particularly nucleotide excision repair (NER), where it facilitates the removal of UV-induced photoproducts and bulky DNA adducts. DDB1 also contributes to the degradation of proteins involved in transcriptional regulation, cell cycle progression, and genome stability. Structurally, DDB1 contains WD40 domains that mediate interactions with CUL4 and substrate receptors, ensuring versatility and specificity in substrate selection. Dysregulation of DDB1 has been associated with genomic instability and cancer development. Its ubiquitous expression highlights its fundamental role in cellular homeostasis and DNA damage response pathways.
Características del producto
category
Primary Antibodies
clonality
monoclonal
reactivity
human,mouse,rat
immunogen target
damage-specific DNA binding protein 1, 127kDa
host
Mouse
isotype
IgG2a
conjugation
Unconjugated
form
liquid
tested applications
ELISA, WB, IHC, IF
observerd MW
127 kDa
purity
≥95% as determined by SDS-PAGE
purification
Protein A+G purification
recommended dilution
WB: 1:500-1:2000; IHC: 1:200-1:800
size 1
100µg
storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
or code
DDB1
alias
XPE,DDBA,XAP1,XPCE,XPE-BF,UV-DDB1,WHIKERS,UV-damaged DNA-binding factor,DNA damage-binding protein a,DDB p127 subunit
uniprot id
note
This product is for research use only.
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