6*His, His-Tag antibody

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935106861
info@markelab.com
name
6*His, His-Tag antibody
category
Primary Antibodies
provider
FineTest
reference
FNab00010
tested applications
ELISA, WB

Description

Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.

Documents del producto

Instrucciones
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Data sheet
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Product specifications

Category
Primary Antibodies
Immunogen Target
(6*His, His-Tag)
Host
Rabbit
Reactivity
Recombinant protein
Recommended Dilution
WB: 1:500-1:3000
Clonality
polyclonal
Conjugation
Unconjugated
Isotype
IgG
Purity
≥95% as determined by SDS-PAGE
Purification
Immunogen affinity purified
Size 1
100µg
Form
liquid
Tested Applications
ELISA, WB
Storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Alias
6*His,6*His,His Tag,6histag,6XHis,HHHHHH,HIS,His Tag,HISTIDINE TAGGED
Background
Antibody anti-6*His, His-Tag
Status
RUO
Note
This product is for research use only.

Descripción

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